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FZD9 antibody (A08165-2) performs well in ELISA, Flow Cytometry, IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of THP-1 cells using anti-Frizzled 9/FZD9 antibody (A08165-2).
Overlay histogram showing THP-1 cells stained with A08165-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with A08165-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Frizzled 9/FZD9 Antibody (A08165-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-Frizzled 9/FZD9 Antibody Picoband®
FZD9 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, WB |
Tested Applications
FZD9 antibody was positively detected by WB in:
rat small intestine tissue, rat testis tissue, mouse small intestine tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
FZD9 antibody was positively detected by IHC in:
human breast cancer tissue, human testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
FZD9 antibody was positively detected by IF in:
human breast cancer tissue, human testis tissue
FZD9 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Human
Immunofluorescence
5 μg/ml
Human
Flow Cytometry (Fixed)
1-3 μg/1x106 cells
Human
ELISA
0.1-0.5 μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Recommend
Mouse
Tested
Recommend
Human
Tested
Tested
Recommend
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Frizzled 9/FZD9 Antibody Picoband®
View all FZD9 Antibodies
View all FZD9 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human Frizzled 9/FZD9 recombinant protein (Position: R27-L591). Human Frizzled 9/FZD9 shares 95.9% and 96.1% amino acid (aa) sequence identity with mouse and rat Frizzled 9/FZD9, respectively.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated Molecular Weight
64.5 kDa
Background of FZD9
Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The FZD9 gene is located within the Williams syndrome common deletion region of chromosome 7, and heterozygous deletion of the FZD9 gene may contribute to the Williams syndrome phenotype. FZD9 is expressed predominantly in brain, testis, eye, skeletal muscle, and kidney.
Cite This Product
Anti-Frizzled 9/FZD9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08165-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Dog
97%
High Homology
Sheep
96%
High Homology
Rat
96%
High Homology
Mouse
96%
High Homology
Rabbit
88%
Moderate Homology
Cow
87%
Moderate Homology
Zebrafish
73%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for FZD9 antibody
Immunohistochemistry (IHC) protocol for FZD9 antibody
Immunofluorescence (IF) protocol for FZD9 antibody
Flow Cytometry (FC) protocol for FZD9 antibody
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Product Promise
We promise Anti-Frizzled 9/FZD9 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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A08165-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 2, 2026