Anti-FRMPD1 Antibody

Product Name

Anti-FRMPD1 Antibody

View all FRMPD1 Antibodies

SKU/Catalog Number

A10887

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-FRMPD1 Antibody (Catalog # A10887). Tested in ELISA, WB, IF applications. This antibody reacts with Human.

Storage & Handling

FRMPD1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-FRMPD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10887)

Host

Rabbit

Contents

FRMPD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

FRMPD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human FRMPD1. The immunogen is located within amino acids 1330 - 1380 of FRMPD1.

Reactive Species

A10887 is reactive to FRMPD1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

173.4 kDa

Background of FRMPD1

The FERM and PDZ domain containing (FRMPD) protein family consists of four proteins that contain a FERM (Four-point-one, erzin, radixin, moesin) domain and at least one PDZ (PSD-95/Discs large/Zonula-occuldens-1) domain. FRMPD1 was initially identified through a yeast two-hybrid screening with Activator of G-protein signaling 3 (AGS3), a protein that acts to stabilize the GDP-bound conformation of Gai, as the bait. FRMPD1 binds to the tetratricopeptide repeat of AGS3 and stabilizes AGS3 in a membrane environment. Suppression of FRMPD1 in Cath.a-differentiated neuronal cells decreased the level of endogenous AGS3 in membrane fractions by ~50% and enhanced the inhibition of forskolin-induced increases in cAMP. Co-immunoprecipitation studies indicate that the interaction of AGS3 with FRMPD1 is mutually exclusive with the interaction of AGS3 with Gai3, suggesting that FRMPD1 may position AGS3 in the membrane where it can then interact with Galphai.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10887 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

FRMPD1 antibody can be used for detection of FRMPD1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information FRMPD1

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