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- Table of Contents
DLG4 antibody (PA2295) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 6 Publications
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IF analysis of PSD95/DLG4 using anti-PSD95/DLG4 antibody (PA2295).
PSD95/DLG4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
PSD95/DLG4 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-PSD95/DLG4 Antibody (PA2295) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG (BA1135) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-PSD95/DLG4 Antibody Picoband®
DLG4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
DLG4 antibody was positively detected by WB in:
rat brain tissue, mouse brain tissue, human U-87MG whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DLG4 antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DLG4 antibody was positively detected by IF in:
rat brain tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Recommend
Human
Tested
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-PSD95/DLG4 Antibody Picoband®
View all DLG4 Antibodies
View all DLG4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human PSD95, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90-100 kDa
Calculated Molecular Weight
80.5 kDa
Background of DLG4
DLG4 discs large homolog 4, also known as PSD95 or SAP-90, is a protein that in humans is encoded by the DLG4 gene. It is a member of the membrane-associated guanylate kinase (MAGUK) family. This gene is mapped to 17p13.1. DLG4 can heteromultimerize with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Overexpression of DLG4 in hippocampal neurons could drive maturation of glutamatergic synapses. DLG4 can orchestrate synaptic development and it has a role in synapse stabilization and plasticity. Ubiquitination of DLG4 through an MDM2-mediated pathway can regulate AMPA receptor surface expression during synaptic plasticity.
Cite This Product
Anti-PSD95/DLG4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2295)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
69%
Low homology
Drosophila
44%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DLG4 antibody
Immunohistochemistry (IHC) protocol for DLG4 antibody
Immunofluorescence (IF) protocol for DLG4 antibody
Publications
Application:
Species:
Electroacupuncture treatment enhances hippocampal growth hormone level and restores mitochondrial function in vascular dementia rats
Published: 2026-06-26
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.20
Species: Rat
PMID: 42361950
Electroacupuncture Promotes Synaptic Recovery After Cerebral Ischemia-Reperfusion by Activating SIRT1 to Inhibit the NF-κB Pathway and Regulate Astrocyte Phenotypic Transformation
Published: 2026-05-18
Journal: NEUROCHEMICAL RESEARCH
Application: IF
Impact Factor: 4.50
Species: Mouse
PMID: 42149357
Chlorpyrifos-induced autism and depression-like behaviors are ameliorated by trehalose: Integrating network pharmacology and toxicology analyses and animal experiments
Published: 2026-01-17
Journal: ENVIRONMENTAL POLLUTION
Application: IF
Impact Factor: 7.30
Species: Mouse
PMID: 41554428
Revealing the effective components and mechanism of Zhimu-Huangbai herb-pair in the treatment of Alzheimer's disease
Published: 2025-03-26
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: IHC
Impact Factor: 5.40
Species: Mouse
PMID: 40154898
Safflower Yellow Alleviates Cognitive Impairment in Mice by Modulating Cholinergic System Function, Oxidative Stress, and CREB/BDNF/TrkB Signaling Pathway
Published: 2024-10-24
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Mouse
PMID: 39461389
IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway
Published: 2024-08-03
Journal: IBRO Neuroscience Reports
Application: IF
Impact Factor: 2.00
Species: Mouse
PMID: 39220229
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Product Promise
We promise Anti-PSD95/DLG4 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA2295
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026