Click image to see more details
Product Info Summary
| SKU: | PA1915 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband®
SKU/Catalog Number
PA1915
BA3097-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Follistatin-related protein 3 FSTL3 Antibody catalog # PA1915. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1915)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human FSTL3, different from the related rat and mouse sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1915 is reactive to FSTL3 in Human, Mouse, Rat
Observed Molecular Weight
22-24 kDa
Calculated molecular weight
27.7 kDa
Background of FSTL3
FSTL3 (Follistatin-Like 3) also known as FLRG or FOLLISTATIN-RELATED GENE, is a member of the follistatin-module protein family, which is composed of extracellular matrix-associated glycoproteins though to act in a paracrine manner to bind morphogens or growth/differentiation factors and regulate their activity during development. The FSTL3 gene extends over 7 kbp and contains 5 exons. By Southern blot analysis of somatic cell hybrids and FISH, Hayette et al. (1998) localized the FSTL3 gene to chromosome 19p13. Using recombinant mouse Fstl3, Tsuchida et al. (2000) found that Fstl3bound both activin and BMP2 and had a higher affinity for activin. Overexpression of Fstl3 inhibited BMP2-induced cell signaling in a reporter assay. In addition to the translocation in a case of B-cell chronic lymphocytic leukemia from which FSTL3 was isolated, Hayette et al. (1998) also observed rearrangement of the FSTL3 gene in a case of B-cell non-Hodgkin lymphoma and in a case of B-cell mantle zone lymphoma, suggesting that FSTL3 may be involved in the leukemogenesis process.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1915 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human U2OS whole cell, human Hela whole cell, human A549 whole cell, human PC-3 whole cell, human HepG2 whole cell, human K562 whole cell, rat liver tissue, rat testis tissue, rat NRK whole cell, mouse liver tissue, mouse testis tissue, mouse lung tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human U2OS whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human HepG2 whole cell lysates,
Lane 6: human K562 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.
Click image to see more details
Expression levels (NPX) of CLSTN2, HGF, SPON1, CRIM1, PLAUR, and FSTL3 in plasma from CTRL and patients with PAH.
According to contemporary cardiac index measured by right heart catheterization at the time blood sample was drawn, patients with PAH were categorized as cRV (cardiac index >2.2 L/[min·m2]) and dRV (cardiac index ≤2.2 L/[min·m2]). Statistical analyses were performed using the Student's t test or 1‐way ANOVA followed by Tukey post hoc test. *P< .05, **P<0.01, ***P<0.001, and ****P<0.0001. CLSTN2 indicates calsyntenin 2; cRV, compensated right ventricle; CRIM1, cysteine rich transmembrane bone morphogenetic protein regulator 1; CTD‐PAH, connective tissue disease‐associated pulmonary arterial hypertension; CTRL, controls; dRV, decompensated right ventricle; FSTL3, follistatin‐like 3; HGF, hepatocyte growth factor; IPAH, idiopathic pulmonary hypertension; NPX, normalized protein expression; PAH, pulmonary arterial hypertension; PLAUR, plasminogen activator, urokinase receptor; and SPON1, spondin 1.
Index in PubMed under a CC BY license. PMID: 38874078
Click image to see more details
Western blot analysis of FSTL3 using anti-FSTL3 antibody (PA1915).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat testis tissue lysates,
Lane 3: rat NRK whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FSTL3 antigen affinity purified polyclonal antibody (Catalog # PA1915) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FSTL3 at approximately 22-24KD. The expected band size for FSTL3 is at 22KD.
Specific Publications For Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband® (PA1915)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Follistatin-related protein 3 FSTL3 Antibody Picoband®
Question
Would anti-FSTL3 antibody PA1915 work on dog WB with left adrenal gland?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
Our lab technicians have not validated anti-FSTL3 antibody PA1915 on dog. You can run a BLAST between dog and the immunogen sequence of anti-FSTL3 antibody PA1915 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog left adrenal gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-07
Question
Would anti-FSTL3 antibody PA1915 work for WB with left adrenal gland?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
According to the expression profile of left adrenal gland, FSTL3 is highly expressed in left adrenal gland. So, it is likely that anti-FSTL3 antibody PA1915 will work for WB with left adrenal gland.
Boster Scientific Support
Answered: 2019-09-11
Question
Can you help my question with product PA1915, anti-FSTL3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1915 anti-FSTL3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-06
Question
I see that the anti-FSTL3 antibody PA1915 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-07-23
Question
We are currently using anti-FSTL3 antibody PA1915 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
The anti-FSTL3 antibody (PA1915) has not been tested for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-06-06
Question
Is a blocking peptide available for product anti-FSTL3 antibody (PA1915)?
T. Mangal
Verified customer
Asked: 2014-04-22
Answer
We do provide the blocking peptide for product anti-FSTL3 antibody (PA1915). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-04-22
