This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
CYGB antibody (A01197) performs well in IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of Cytoglobin using anti-Cytoglobin antibody (A01197).
Cytoglobin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytoglobin Antibody (A01197) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Cytoglobin was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cytoglobin Antibody (A01197) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Designing a Western blot or IHC for CYGB? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
Bundle Save
Anti-Cytoglobin/CYGB Antibody Picoband®
CYGB antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IHC, WB |
Tested Applications
CYGB antibody was positively detected by WB in:
rat small intestine tissue, mouse small intestine tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
CYGB antibody was positively detected by IHC in:
mouse intestine tissue, mouse cardiac muscle tissue, rat intestine tissue, human intestinal cancer tissue, human lung cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Mouse, Rat
Western blot
0.1-0.5μg/ml
Mouse, Rat, Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Tested
Recommend
Human
Tested
Recommend
Sheep
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Cytoglobin/CYGB Antibody Picoband®
View all CYGB Antibodies
View all CYGB Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cytoglobin recombinant protein (Position: M1-P190). Human Cytoglobin shares 95.3% and 93.7% amino acid (aa) sequence identity with mouse and rat Cytoglobin, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
21.4 kDa
Background of CYGB
Cytoglobin (CYGB), also called HGB or STAP, is a ubiquitously expressed hexacoordinate hemoglobin that may facilitate diffusion of oxygen through tissues, scavenge nitric oxide or other reactive oxygen species, or serve a protective function during oxidative stress. The cytoglobin gene is mapped on 17q25.1. The CYGB gene contains 4 exons and spans about 9 kb. Cytoglobin has many elements common to vertebrate globins, including invariant histidine residues, and the amino acids that form the heme pocket share similarity with pentacoordinate myoglobin. In contrast to the high oxygen affinities displayed by most hexacoordinate hemoglobins, the characteristics of CYGB indicate that it can facilitate oxygen transport. Because the oxygen affinity of CYGB is more similar to myoglobin than to neuroglobin, and the oxy form of CYGB resists autooxidation, CYGB is proposed to represent a tissue oxygen reservoir.
Cite This Product
Anti-Cytoglobin/CYGB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01197)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Sheep
98%
High Homology
Cow
97%
High Homology
Dog
96%
High Homology
Mouse
95%
High Homology
Rat
94%
High Homology
Monkey
93%
High Homology
Rabbit
72%
Moderate Homology
Zebrafish
64%
Low homology
Drosophila
23%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for CYGB antibody
Immunohistochemistry (IHC) protocol for CYGB antibody
Review
Have you used Anti-Cytoglobin/CYGB Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-Cytoglobin/CYGB Antibody Picoband® performs excellently in relevant applications for IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01197
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 16, 2026