Human Renin / Prorenin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Renin. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human Renin / Prorenin ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Sample Volume:||100μl per well|
|Product Name||Human Renin / Prorenin ELISA Kit PicoKine™
See all Renin primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Renin/REN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Renin/REN tissue specificity:|
|Cite This Product||Human Renin / Prorenin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1249)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: L67-R406|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For REN (Source: Uniprot.org, NCBI)
|NCBI Gene Id||5972|
|Species Of This Entry||Human|
|Superfamily||peptidase A1 family|
|Alternative Names||Renin|angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 220.127.116.11; FLJ10761; HNFJ2; REN; renin precursor, renal; Renin|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Ubiquitination; Glycosylation; Deacetylation; Acetylation; Oxidation; Methylation; Dephosphorylation; Biotinylation; Reduction; Deglycosylation; Myristoylation|
|Subcellular Localization||Secreted. Membrane. Associated to membranes via binding to ATP6AP2.|
|Gene Location||1q32.1, on Chromasome 1, gene sequence: NC_000001.11 (204154819..204166337, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For REN (Source: Uniprot.org, NCBI)
|Protein Function||Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.|
|Related Diseases||Hypertensive Disease; Neoplasms; Malignant Neoplasms; Mesothelioma; Malignant Paraganglionic Neoplasm; Kidney Diseases; Essential Hypertension; Malignant Mesothelioma; Cell Transformation, Neoplastic; Pleural Neoplasms; Hypertrophy; Medulloblastoma; Hemorrhage; Carcinoma; Nervousness; Pleural Mesothelioma; Malignant Pleural Mesothelioma; Dysequilibrium Syndrome; Cardiovascular Diseases; Malignant Neoplasm Of Breast|
|Related Pathways||Pathogenesis; Localization; Secretion; Transport; Cell Proliferation; Excretion; Cell Death; Oncogenesis; Glycosylation; Ovulation; Aging; Translation; Proprioception; Vasodilation; Detection Of Glucose; Ossification; Tropism; Reflex; Cell Differentiation; Chromatin Remodeling|
|Background||Renin, also known as an angiotensinogenase, is an enzyme that participates in the body's renin-angiotensin system(RAS). This gene is mapped to 1q32.1. Renin's primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. What's more, Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation.|
|Scientific References||PMID: 6324167 by Imai T., et al. Cloning and sequence analysis of cDNA for human renin precursor.|
PMID: 3530608 by Morris B.J.; New possibilities for intracellular renin and inactive renin now that the structure of the human renin gene has been elucidated.
Kit Components And Assay QC Data Details Of Human Renin / Prorenin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1249-CAP||96-well plate precoated with anti-Human Renin antibody||1|
|EK1249-ST||lyophilized recombinant Human Renin standard||10ng/tube|
|EK1249-DA||biotinylated anti-Human Renin antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1249 Human Renin / Prorenin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human Renin / Prorenin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Renin / Prorenin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 1
|Wang C, Ren X. Hybridoma (Larchmt). 2010 Dec;29(6):511-6. Doi: 10.1089/Hyb.2010.0044. Epub 2010 Nov 18. Preparation And Characterization Of Polyclonal Antibody Against Severe Acute Respiratory Syndrome-Associated Coronavirus Spike Protein.||PubMed ID 21087096|
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