Product Info Summary
| SKU: | A01907 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-GABARAP Antibody
SKU/Catalog Number
A01907
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GABARAP Antibody (Catalog # A01907). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
GABARAP antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-GABARAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01907)
Host
Rabbit
Contents
GABARAP Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
GABARAP antibody was raised against a 19 amino acid peptide near the amino terminus of human GABARAP. The immunogen is located within the first 50 amino acids of GABARAP.
Cross-reactivity
Multiple isoforms of GABARAP exist due to alternative splicing events.
Reactive Species
A01907 is reactive to GABARAP in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
13.9 kDa
Background of GABARAP
Gamma-aminobutyric acid (GABA) is the main inhibitory transmitter by increasing a Cl-conductance that inhibits neuronal firing in the central nervous system. It has been shown to activate both ionotropic (GABAA) and metabotropic (GABAB) receptors as well as a third class of receptors called GABAC. GABARAP (GABAA receptor-associated protein) links GABAA receptors to the cytoskeleton and may play a role in intracellular transport of GABAA receptors and its interaction with the cytoskeleton. GABARAP belongs to the MAP1 or ATG8 like family and recent studies show that MAPK15/ERK8 is acting through interaction with ATG8 family proteins to regulate autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01907 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
GABARAP antibody can be used for detection of GABARAP by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of GABARAP in HeLa cell lysate with GABARAP antibody at 1 μg/ml.
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Immunofluorescence of GABARAP in human brain tissue with GABARAP antibody at 20 μg/ml.
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Immunohistochemistry of GABARAP in mouse brain tissue with GABARAP Antibody at 5 μg/mL.
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Immunofluorescence of GABARAP in mouse brain tissue with GABARAP Antibody at 20 μg/mL.
Green: GABARAP antibody (A01907)
Red: Phylloidin staining
Blue: DAPI staining
Specific Publications For Anti-GABARAP Antibody (A01907)
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