Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12)

MCAM/CD146 antibody

Boster Bio Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12) catalog # M01683-3. Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M01683-3
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, IHC-F, WB

Product Name

Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12)

View all MCAM/CD146 Antibodies

SKU/Catalog Number

M01683-3

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12) catalog # M01683-3. Tested in Flow Cytometry, IHC, IHC-F, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01683-3)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

4C12

Isotype

Mouse IgG2a

Immunogen

E.coli-derived human CD146 recombinant protein (Position: H59-A401). Human CD146 shares 73% amino acid (aa) sequence identity with both mouse and rat CD146.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M01683-3 is reactive to MCAM in Human

Applications

M01683-3 is guaranteed for Flow Cytometry, IHC, IHC-F, WB Boster Guarantee

Observed Molecular Weight

120 kDa

Calculated molecular weight

71.607kDa

Background of MCAM/CD146

CD146 (cluster of differentiation 146), also known as the melanoma cell adhesion molecule (MCAM) or cell surface glycoprotein MUC18, is a 113kDa cell adhesion molecule currently used as a marker for endothelial cell lineage. MCAM, a member of the immunoglobulin superfamily, is homologous to several cell adhesion molecules and is associated with tumor progression and the development of metastasis in human malignant melanoma. By radiation hybrid analysis, this gene is mapped to chromosome 11q23.3. MCAM has been demonstrated to appear on a small subset of T and B lymphocytes in the peripheral blood of healthy individuals. MCAM has been seen as a marker for mesenchymal stem cells isolated from multiple adult and fetal organs, and its expression may be linked to multipotency mesenchymal stem cells with greater differentiation potential express higher levels of MCAM on the cell surface.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For MCAM (Source: Uniprot.org, NCBI)

Gene Name

MCAM

Full Name

Cell surface glycoprotein MUC18

Weight

71.607kDa

Alternative Names

CD146 antigen; CD146; cell surface glycoprotein MUC18; Cell surface glycoprotein P1H12; L-Gicerin; MCAM; melanoma adhesion molecule; melanoma cell adhesion moleculeS-endo 1 endothelial-associated antigen; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; MUC18; MUC18Gicerin MCAM CD146, HEMCAM, METCAM, MUC18, MelCAM melanoma cell adhesion molecule cell surface glycoprotein MUC18|Gicerin|S-endo 1 endothelial-associated antigen|cell surface glycoprotein P1H12|melanoma adhesion molecule|melanoma-associated antigen A32|melanoma-associated antigen MUC18

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on MCAM, check out the MCAM Infographic

MCAM infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for MCAM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M01683-3

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12)?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12)

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

0 Customer Q&As for Anti-CD146/MCAM Antibody Picoband™ (monoclonal, 4C12)

Order DetailsPrice
M01683-3

100μg

$370
M01683-3-10ug

10μg sample (liquid)

$99
M01683-3-Biotin

100 μg Biotin conjugated

$570
M01683-3-Cy3

100 μg Cy3 conjugated

$570
M01683-3-Dylight488

100 μg Dylight488 conjugated

$570
M01683-3-Dylight550

100 μg Dylight550 conjugated

$570
M01683-3-Dylight594

100 μg Dylight594 conjugated

$570
M01683-3-FITC

100 μg FITC conjugated

$570
M01683-3-HRP

100 μg HRP conjugated

$570
M01683-3-APC

100 μg APC conjugated

$670
M01683-3-PE

100 μg PE conjugated

$670
M01683-3-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
M01683-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.