Anti-GANP MCM3AP Antibody

Product Name

Anti-GANP MCM3AP Antibody

View all MCM3AP Antibodies

SKU/Catalog Number

A05445

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-GANP MCM3AP Antibody (Catalog # A05445). Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GANP MCM3AP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05445)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human GANP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05445 is reactive to MCM3AP in Human, Mouse

Reconstitution

Observed Molecular Weight

111 kDa

Calculated molecular weight

218405 MW

Background of MCM3AP

Isoform GANP:Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells By similarity. Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Isoform MCM3AP:Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.

Nobuo Sakaguchi, J. Immunol., Apr 2005; 174: 4485 - 4494.
Kazuhiko Kuwahara, Blood, Apr 2000; 95: 2321 - 2328.
Y Kono, K Maeda, Genes Cells, Aug 2002; 7: 821 - 834.
Kazuhiko Kuwahara, PNAS, Aug 2001; 98: 10279.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05445 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100

Positive Control

Validation Images & Assay Conditions

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Western blot analysis of extracts from HepG2 cells (Lane 2) and A549 cells (Lane 3), using GANP antiobdy A05445. The lane on the left is treated with synthesized peptide.

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Western blot analysis of extracts from NIH/3T3 cells, using GANP antibody A05445.

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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GANP antibody A05445.

Gene/Protein Information For MCM3AP (Source: Uniprot.org, NCBI)

Alternative Names

80 kDa MCM3-associated protein; FLJ44336; GANPFLJ45306; human mRNA for MCM3 import factor10Protein GANP; KIAA0572MAP80germinal center-associated nuclear protein; Map80; MCM3 import protein; MCM3 minichromosome maintenance deficient 3 (S. cerevisiae) associated protein; MCM3 minichromosome maintenance deficient 3 associated protein; minichromosome maintenance complex component 3 associated protein; minichromosome maintenance deficient (S. cerevisiae) 3-associated protein; minichromosome maintenance deficient 3-associated protein MCM3AP GANP, MAP80, PNRIID, SAC3 minichromosome maintenance complex component 3 associated protein germinal-center associated nuclear protein|80 kDa MCM3-associated protein|MCM3 acetylating protein|MCM3 acetyltransferase|MCM3 import protein|MCM3 minichromosome maintenance deficient 3 associated protein|germinal center-associated nuclear protein|germinal-centre associated nuclear protein

For more info on MCM3AP, check out the MCM3AP Infographic

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In this infographic, you will see the following information for MCM3AP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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