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Product Info Summary
| SKU: | A07210 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-GCN5 KAT2A Antibody
SKU/Catalog Number
A07210
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GCN5 KAT2A Antibody catalog # A07210. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCN5 KAT2A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07210)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GCN5L2. AA range:691-740
Reactive Species
A07210 is reactive to KAT2A in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
93.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07210 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of Hela cells using GCN5 Polyclonal Antibody diluted at 1:1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit .
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Western blot analysis of the lysates from RAW264.7cells using GCN5L2 antibody.
Specific Publications For Anti-GCN5 KAT2A Antibody (A07210)
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Customer Q&As
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7 Customer Q&As for Anti-GCN5 KAT2A Antibody
Question
Is a blocking peptide available for product anti-GCN5 antibody (A07210)?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
We do provide the blocking peptide for product anti-GCN5 antibody (A07210). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-03-30
Question
Would A07210 anti-GCN5 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
It shows on the product datasheet, A07210 anti-GCN5 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use your anti-GCN5 antibody for WB for mouse liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-02-28
Answer
As indicated on the product datasheet, A07210 anti-GCN5 antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-28
Question
I have attached the WB image, lot number and protocol we used for liver using anti-GCN5 antibody A07210. Please let me know if you require anything else.
K. Williams
Verified customer
Asked: 2018-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-13
Question
My question regarding product A07210, anti-GCN5 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07210 anti-GCN5 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-05
Question
Does anti-GCN5 antibody A07210 work on pig WB with brain?
A. Roberts
Verified customer
Asked: 2016-10-27
Answer
Our lab technicians have not validated anti-GCN5 antibody A07210 on pig. You can run a BLAST between pig and the immunogen sequence of anti-GCN5 antibody A07210 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-10-27
Question
Is this A07210 anti-GCN5 antibody reactive to the isotypes of KAT2A?
A. Li
Verified customer
Asked: 2013-11-12
Answer
The immunogen of A07210 anti-GCN5 antibody is Synthesized peptide derived from the C-terminal region of human GCN5. at AA rangle: 660-740. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-11-12
