KAT2A Antibodies

Histone acetyltransferase KAT2A (KAT2A)

Protein lysine acyltransferase that could act both as a acetyltransferase and succinyltransferase, depending on the context (PubMed:29211711). Succinylation of histones gives a specific tag for epigenetic transcription activation (PubMed:29211711).

Association with the 2-oxoglutarate dehydrogenase complex, which provides succinyl-CoA, is required for histone succinylation (PubMed:29211711). In various complexes, functions either as an acetyltransferase (HAT) or as a succinyltransferase: at the SAGA and ATAC complexes, functions as a histone acetyltransferase (PubMed:17301242, PubMed:19103755, PubMed:29211711).

Gene Information

Human
Gene Name:	KAT2A
Uniprot:	Q92830
Entrez:	2648

Family

Belongs to:
acetyltransferase family

Alternative Names

EC 2.3.1.48; GCN5 general control of amino-acid synthesis 5-like 2 (yeast); GCN5L2GCN5 (general control of amino-acid synthesis, yeast, homolog)-like 2; GCN5MGC102791; General control of amino acid synthesis protein 5-like 2; General control of amino acid synthesis, yeast, homolog-like 2; hGCN5; Histone acetyltransferase GCN5; histone acetyltransferase KAT2A; hsGCN5; K(lysine) acetyltransferase 2A; Lysine acetyltransferase 2A; PCAF-b; STAF97

Molecular Weight

Mass (kDA):
93.926 kDA

Genomic Context

Human
Location:	17q21.2
Sequence:	17; NC_000017.11 (42113111..42121367, complement)

Tissue Specificity

Expressed in all tissues tested, with most abundant expression in ovary.

Subcellular Localizations

Nucleus. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Mainly localizes to the nucleus (PubMed:27796307). Also localizes to centrosomes in late G1 and around the G1/S transition, coinciding with the onset of centriole formation (PubMed:27796307).

Diseases

• Malignant Neoplasms
• Metabolic Diseases
• Metaplastic Cell Transformation
• Malignant Neoplasm Of Breast
• Ataxia
• Neoplasms
• Spinocerebellar Ataxia Type 7
• Cell Transformation, Neoplastic
• Diabetes Mellitus
• Virus Diseases
• Diabetes Mellitus, Non-insulin-dependent
• Ataxia, Spinocerebellar
• Cystic Fibrosis

• Retinal Degeneration
• Neurodegenerative Disorders
• Trinucleotide Repeat Expansion
• Immunologic Deficiency Syndromes
• Osteosarcoma
• Acute Erythroblastic Leukemia
• Fibrosis

Interacting Proteins

• AATF
• E2F1
• WDHD1
• ZZZ3

Pathways

• Histone Acetylation
• Cell Cycle
• Gluconeogenesis
• Cell Proliferation
• Cell Growth
• Rna Interference
• Transport
• Histone Deacetylation
• Cellular Homeostasis
• Aging
• Response To Virus
• Regulation Of Gene Expression
• Energy Homeostasis

• Senescence
• Replicative Senescence
• Mismatch Repair
• Protein Acetylation
• S Phase
• Chromatin Modification
• Fatty Acid Oxidation

PTMs

• Acetylation
• Phosphorylation

• Deacetylation
• Dephosphorylation

• Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/KAT2A

Boster Bio - Best Uses Of The KAT2A Marker

This article explains the Boster Bio AntigCN5 KAT2A Marker. It also discusses SGO1 AS1 lentiviral vectors, PTBP1 short hair RNA (shRNA) vectors and high-affinity primary antibodies. They can be used for various purposes and samples. They are also useful to scientists across the globe.

Boster Bio Anti-GCN5 KAT2A Marker

The Boster Bio Anti–GCN5 Cat2A marker is an antigen monoclonal. It was developed and validated to be used in Immunohistochemistry or Western Blotting applications. Its high affinity enables it to recognize GCN5 and its target proteins, while its specificity is essential for successful IHC. The methods that the Boster Anti-GCN5 KAT2A Marker uses conforms to the specifications listed on the data sheet for the product.

SGO1-AS1 lentiviral vectors

Boster Bio SGO1-AS1 and SGO2 lentiviral vectors are designed to inhibit and target the expression of SGO1 and SGO2. In this study, lentiviRAL SGO1-AS1 vectors were used to enhance the gene and knock down SGO2 with a siRNA targeting ZEB1. Both SGO1 and SGO2 are key cancer genes, and the overexpression of either one of these proteins reduced the risk of lung metastases.

SGO1-AS1 lentiviRAL Vectors impeded cell invasion and migration in SGC7901 and BGC823 cells. SGO1 AS1 knockdown increased MKN28 cells migration and invasion. Moreover, overexpression of SGO1-AS1 reduced the growth of long-term cells while knockdown did not affect short-term cell growth. SGO1-AS1 overexpression inhibited soft agar colony formation. The opposite effect was observed and colony size increased after silencing this gene.

In vitro and in vivo SGO1-AS1 is a potent inhibitor of GC invasion and metastasis. SGO1-AS1 was shown to bind to G3BP2 and other proteins.

SGO1-AS1 lentivirial vectors target the TGFB mRNA that competes with TGFB1/2 for binding to PTBP1. By targeting the PTBP1 gene, SGO1-AS1 blocks TGFB mRNA decay which in turn reduces TGFb protein expression. This effect can be reversed when PTBP1 is co-expressed.

SGO1-AS1 was cotransfected using packaging plasmids from HEK293T cell lines to determine the expression of PTBP1. Infections of MKN28 cells using these lentiviral vectors were conducted and single cells taken out 48 hours after infection. Single cells were cultured for 3 weeks before being analyzed for PTBP1 knockout or expression levels.

PTBP1 short hair RNA (shRNA) lentiviral vectors

A new generation of lentiviral vectors that target PTBP1 contains shRNAs that target conserved regions of the cellular protein. These lentiviral vectors could safeguard humans from two devastating neurodegenerative diseases: scrapie as well as Japanese encephalitis (JE). These vectors have been found to decrease PrpC levels in transgenic mice, thereby increasing the survival of these animals when infected by the prion strain of the scrapie virus.

The PTBP1 shRNA lentiviral vectors of Boster bio are engineered to deliver single, conditional, and multiple shRNAs within the same cell line. The lentiviral vector is able to deliver multiple shRNAs by using the pSLIK program. The researchers used this system to knock down the human oncogene Ga12, which is associated with the growth of tumors.

The vectors lentiviral contained anti-HIV shRNAs that targeted the gag and pol genes in wild-type HIV-1. The packaging construct divides the cod cod coding region into two expression cassettes. This reduces the risk of generating a replication competent virus through non-homologous mutation.

Recent studies have revealed that mature miRNAs can have extremely variable ends. These variations could be due to a lack of precision in the Drosha/Dicer processing process or changes after miRNA synthesizing. These results do not correspond to the experimental results for miR-30. However, further data about miRNA biogenesis could be helpful in the development of a more rational shRNA design.

These new lentivirus vectors comprise shRNA that targets TNF alpha. In vitro they inhibit the expression of TNF-alpha as well as osteolysis. Additionally these lentiviral vectors could be utilized for gene therapy studies. They are effective in transfecting macrophages. They were able to achieve 85% efficiency at a MOI of just 150.

Primary antibodies with high affinity

Boster Bio is a great place to purchase high-affinity antibody. Since its inception in 1993, the company has developed hundreds of different primary antibodies for use for a variety of purposes, including IHC and Western blotting. In the latter half of the 1990s, Boster had become one of the most prominent catalog antibody producers in China. Boster's own ELISA platform, PicoKine(tm), was designed to provide highly sensitive ELISA kits to researchers.

Boster's primary antibody are therefore highly specific and reliable. They are widely used in biomedical research, and have received numerous citations. They've been validated in Western blotting, Immunohistochemistry, and ELISA, allowing researchers to use them confidently in their research. They're compatible with a variety of research workflows, from single-cell to large-scale multi-laboratory settings.

Besides offering primary antibodies, Boster Bio also offers a variety of other reagents, including buffers, lysates ELISA kits and fusion proteins. The Boster Rabbit polyclonal antibodies are extremely specialized and have been proven to be suitable for use in neurosciences, cancer biology, and development biology. All products are subjected rigorous testing and are supported by quality assurance.

After securing clones that had a high specificity, DNA insertions containing heavy and light chains of antibodies were sequenced. The heavy chains from the selected unique clones were subcloned into an expression vector in mammalian. ExpiCHO cells generated full-length rabbit IgG1 clones. The Wikipedia page provides more details. These antibodies can be utilized in clinical trials as well as in research laboratories that require high-affinity primary antibodies.

Monoclonal high-affinity monoclonal antigens are distinguished by their high affinity for specific antigens. They can be utilized in various immunoassays, such as WB, ELISA or Flow cytometry. Negative controls are also suggested to verify their specificity. They are used to determine whether an additional antibody is binding not specifically to an antigen. False positives are also identified by negative controls.