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Product Info Summary
| SKU: | PB9713 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-KAT2A/GCN5 Antibody Picoband®
SKU/Catalog Number
PB9713
PB0753 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-KAT2A/GCN5 Antibody Picoband® catalog # PB9713. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-KAT2A/GCN5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9713)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human KAT2A/GCN5, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9713 is reactive to KAT2A in Human
Observed Molecular Weight
94 kDa
Calculated molecular weight
93.9 kDa
Background of KAT2A
Histone acetyltransferase KAT2A is an enzyme that in humans is encoded by the KAT2A gene. It is mapped to 17q21. KAT2A, or GCN5, GCN5L2, is a histone acetyltransferase (HAT) that functions primarily as a transcriptional activator. The GCN5 protein, a regulator of transcription activation in yeast, promotes maximal levels of transcription by 2 transcriptional activators, GCN4 and the HAP2-HAP3-HAP4 complex. The GCN4 protein activates transcription of a large number of amino acid biosynthetic genes under limiting amino acid conditions and the HAP2-HAP3-HAP4 complex is thought to mediate transcription of genes involved in respiratory functions. GCN5 also functions as a repressor of NF-kappa-B by promoting ubiquitination of the NF-kappa-B subunit RELA in a HAT-independent manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9713 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A431 Whole Cell, 22RV1 Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of KAT2A/GCN5 using anti-KAT2A/GCN5 antibody (PB9713).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40ug of sample under reducing conditions.
Lane 1: A431 Whole Cell Lysate,
Lane 2: 22RV1 Whole Cell Lysate,
Lane 3: COLO320 Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT2A/GCN5 antigen affinity purified polyclonal antibody (Catalog # PB9713) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT2A/GCN5 at approximately 94 kDa. The expected band size for KAT2A/GCN5 is at 94 kDa.
Specific Publications For Anti-KAT2A/GCN5 Antibody Picoband® (PB9713)
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1 Customer Q&As for Anti-KAT2A/GCN5 Antibody Picoband®
Question
We are currently using anti-KAT2A/GCN5 antibody PB9713 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on pig tissues as well?
L. Wu
Verified customer
Asked: 2016-07-14
Answer
The anti-KAT2A/GCN5 antibody (PB9713) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-07-14
