This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
GUCY1B1 antibody (A32396-1) performs well in ELISA, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of GUCY1B3 using anti-GUCY1B3 antibody (A32396-1).
GUCY1B3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GUCY1B3 Antibody (A32396-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
GUCY1B3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:200 rabbit anti-GUCY1B3 Antibody (A32396-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-GUCY1B3/GUCY1B1 Antibody
GUCY1B1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, IP, IF, IHC, ICC, WB |
Tested Applications
GUCY1B1 antibody was positively detected by WB in:
human SH-SY5Y whole cell lysates, human U251 whole cell lysates, human HEL whole cell lysates, rat lung tissue lysates, rat brain tissue lysates, mouse lung tissue lysates, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GUCY1B1 antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-400
Immunocytochemistry/Immunofluorescence
1:50-400
Immunoprecipitation
1:250-300
ELISA
1:100-1000
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
IP
ELISA
Human
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-GUCY1B3/GUCY1B1 Antibody
View all GUCY1B1 Antibodies
View all GUCY1B1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human GUCY1B1 recombinant protein (Position: 284-616).
Observed Molecular Weight
71 kDa
Calculated Molecular Weight
70.5 kDa
Background of GUCY1B1
This gene encodes the beta subunit of the soluble guanylate cyclase (sGC), which catalyzes the conversion of GTP (guanosine triphosphate) to cGMP (cyclic guanosine monophosphate). The encoded protein contains an HNOX domain, which serves as a receptor for ligands such as nitric oxide, oxygen and nitrovasodilator drugs. Alternative splicing results in multiple transcript variants.
Cite This Product
Anti-GUCY1B3/GUCY1B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32396-1)
Protocols
Western Blot protocol for GUCY1B1 antibody
Immunohistochemistry (IHC) protocol for GUCY1B1 antibody
Review
Have you used Anti-GUCY1B3/GUCY1B1 Antibody?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-GUCY1B3/GUCY1B1 Antibody performs excellently in relevant applications for ELISA, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A32396-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026