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GFAP antibody (PA1239) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 195 Publications

IHC analysis of GFAP using anti-GFAP antibody (PA1239).
GFAP was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-GFAP Antibody (PA1239) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Anti-GFAP Antibody Picoband®

Name: Anti-GFAP Antibody Picoband®
Brand: Boster Bio
SKU: PA1239
Price: 370 USD
Availability: InStock
Rating: 100 (2 reviews)

GFAP antibody

195 Publications|

2 Reviews

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Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	IF, IHC, WB

Tested Applications

GFAP antibody was positively detected by WB in:

human U251 whole cell, rat brain tissue, mouse brain tissue

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

GFAP antibody was positively detected by IHC in:

rat brain tissue, mouse brain tissue, human brain tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

GFAP antibody was positively detected by IF in:

mouse brain tissue

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Recommend

Rat

Tested

Recommend

Mouse

Tested

Rabbit

Predicted

Bovine

Predicted

Sheep

Predicted

Monkey

Predicted

Dog

Predicted

Product Information

Product Name

Anti-GFAP Antibody Picoband®
View all GFAP Antibodies

Form

Lyophilized

Uniprot

P14136

Gene ID

2670

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP, identical to the related rat sequence, and different from the related mouse sequence by two amino acids.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated Molecular Weight

49.9 kDa

Background of GFAP

Glial fibrillary acidic protein (GFAP) is an intermediate-filament (IF) protein that is highly specific for cells of astroglial lineage, although its tissue-specific role is speculative. GFAP has been located in rat kidney glomeruli and peritubular fibroblasts, leydig cells of testis, skin keratinocytes, osteocytes of bones, chondrocytes of epiglottis, bronchus, and stellate-shaped cells of the pancreas and liver. Its expression is essential for normal white matter architecture and blood-brain barrier integrity, and its absence leads to late-onset CNS dysmyelination. GFAP has also been shown to play a role in mitosis by adjusting the filament network present in the cell. During mitosis, there is an increase in the amount of phosphorylated GFAP, and a movement of this modified protein to the cleavage furrow.

Cite This Product

Anti-GFAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1239)

Protocols

Western Blot protocol for GFAP antibody

Review Protocol

Immunohistochemistry (IHC) protocol for GFAP antibody

Review Protocol

Immunofluorescence (IF) protocol for GFAP antibody

Review Protocol

Publications

Enhanced affinity for lipophilic dyes in neurons damaged by ischemia or hypoxia

Published: 2026-04-23

Journal: JOURNAL OF NEUROSCIENCE METHODS

Application: IF

Impact Factor: 2.30

Species: Rat

PMID: 42034126

SKU	PA1239
Application	Immunofluorenscence
Sample	Rat Brain
Sample Processing Description	Fixed in 4% paraformaldehyde for 48 hours, followed by paraffin embedding and sectioning.
Primary Antibody	Anti-GFAP antibody
Primary Incubation	1:500, overnight at 4 ℃
Blocking Agent	Goat serum
Secondary Antibody	DyLight 550-conjugated goat anti-rabbit antibody.
Secondary Incubation	Incubate at room temperature for 1 hour
Detection	Laser confocal microscopy
Results Summary	During the experiment, this primary antibody can be conveniently used with the complimentary antibody diluent provided. The results obtained showed good quality and reproducibility, with no false-positive signals. Overall, this primary antibody offers excellent cost performance and provides key data and strong support for publication.

SKU	PA1239
Applications	Immunohistochemistry
Sample	Spinal cord
Detection	Confocal microscope

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Anti-GFAP Antibody Picoband®

GFAP antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Enhanced affinity for lipophilic dyes in neurons damaged by ischemia or hypoxia

Neural stem cells transplantation combined with ethyl stearate promotes the activation of quiescent NSCs and improves PD rats motor behavior

Intranasal triptolide in mice: a strategy to reduce neuroinflammation and boost cognitive function

Food-borne polystyrene microplastic exposure exacerbates cognitive deficiency via enhanced neuronal synaptic damage and neuroinflammation in Alzheimer's disease

Precisely targeting of engineered nanovesicles to implanted scaffolds via click chemistry for microenvironment regulation in acute spinal cord injury

Targeted ErbB4 receptor activation ameliorates neuronal deficits via DOCK3 signaling in a transgenic mouse AD model

Surgical-Adjuvant Hydrogel of Full-Natural Bioactive Compounds from Chinese Medicine for Enhanced Traumatic Brain Injury Therapy by Inhibiting Complement Activation

Salvianolic Acid B and Ginsenoside Rg1 Combination Attenuates Cerebral Edema Accompanying Glymphatic Modulation

Low-Frequency (1 Hz) Repetitive Trans-Spinal Magnetic Stimulation Attenuates NLRP3-Driven Neuroinflammation and Enhances Motor Recovery in Spinal Cord-Injured Mice

Neuregulin 2 reversed astrocytosis in the spinal cord in a mouse model of Alzheimer’s disease

Tauroursodeoxycholic acid regulates macrophage/monocyte distribution and improves spinal microenvironment to promote nerve regeneration through inhibiting NF-κB signaling pathway in spinal cord injury

An engineering-reinforced extracellular vesicle–integrated hydrogel with an ROS-responsive release pattern mitigates spinal cord injury

Targeted activation of ErbB4 receptor ameliorates neuronal deficits and neuroinflammation in a food-borne polystyrene microplastic exposed mouse model

Early functional and structural hippocampal impairment in a bilateral common carotid artery stenosis mouse model

Local delivery of mesenchymal stem cell-extruded nanovesicles through a bio-responsive scaffold for acute spinal cord injury treatment

Chitosan-Modified Hydrogel Microsphere Encapsulating Zinc-Doped Bioactive Glasses for Spinal Cord Injury Repair by Suppressing Inflammation and Promoting Angiogenesis

Bu Shen Huo Xue Formula Provides Neuroprotection Against Spinal Cord Injury by Inhibits Oxidative Stress by Activating the Nrf2 Signaling Pathway

The impact of ICOSL/ICOS pathway-regulated long non-coding RNAs on liver fibrosis in mice infected with Schistosoma japonicum

Pathological polarizations from microglia to astrocyte contributes to spatial memory deficit in methamphetamine abstinence mice

Low frequency of repetitive trans-spinal magnetic stimulation promotes functional recovery after spinal cord injury in mice through inhibiting TGF-β1/Smad2/3 signaling pathway

A neurotherapeutic approach with Lacticaseibacillus rhamnosus E9 on gut microbiota and intestinal barrier in MPTP-induced mouse model of Parkinson’s disease

Transplantation of Wnt5a-modified Bone Marrow Mesenchymal Stem Cells Promotes Recovery After Spinal Cord Injury via the PI3K/AKT Pathway

Menthol-Modified Quercetin Liposomes with Brain-Targeting Function for the Treatment of Senescent Alzheimer’s Disease

GSDMD/Drp1 signaling pathway mediates hippocampal synaptic damage and neural oscillation abnormalities in a mouse model of sepsis-associated encephalopathy

Sevoflurane Alters Serum Metabolites in Elders and Aging Mice and Increases Inflammation in Hippocampus

Borneol-Modified Schisandrin B Micelles Cross the Blood–Brain Barrier To Treat Alzheimer’s Disease in Aged Mice

Transcriptional and epigenetic dysregulation impairs generation of proliferative neural stem and progenitor cells during brain aging

Tauroursodeoxycholic Acid Inhibited Apoptosis and Oxidative Stress in H2O2-Induced BMSC Death via Modulating the Nrf-2 Signaling Pathway: the Therapeutic Implications in a Rat Model of Spinal Cord Injury

Transplantation of MiR-28-5p-Modified BMSCs Promotes Functional Recovery After Spinal Cord Injury

The neuroprotective effects of Liuwei Dihuang medicine in the APP/PS1 mouse model are dependent on the PI3K/Akt signaling pathway

Cotransplantation of NSCs and ethyl stearate promotes synaptic plasticity in PD rats by Drd1/ERK/AP-1 signaling pathway

Regulation of Microglial Activation by Wnt/β-Catenin Signaling After Global Cerebral Ischemia in Mice

Betulinic acid alleviates neuropathic pain induced by chronic constriction injury of the sciatic nerve in mice

Nanozyme-Integrated Thermoresponsive In Situ Forming Hydrogel Enhances Mesenchymal Stem Cell Viability and Paracrine Effect for Efficient Spinal Cord Repair

CXCR2 antagonism attenuates neuroinflammation after subarachnoid hemorrhage

Injectable hydrogel microspheres encapsulating extracellular vesicles derived from melatonin-stimulated NSCs promote neurogenesis and alleviate inflammation in spinal cord injury

Measurement of Patient-Derived Glioblastoma Cell Response to Temozolomide Using Fluorescence Lifetime Imaging of NAD(P)H

Inflachromene attenuates seizure severity in mouse epilepsy models via inhibiting HMGB1 translocation

Sex Differences in the Expression of Neuroimmune Molecules in the Spinal Cord of a Mouse Model of Antiretroviral-Induced Neuropathic Pain

Engineering of M2 Macrophages-Derived Exosomes via Click Chemistry for Spinal Cord Injury Repair

ROS filter coating scaffold protects 3D mesenchymal stem cell spheroids for dual-phase treatment of spinal cord injury

Engineered exosomes enriched in netrin-1 modRNA promote axonal growth in spinal cord injury by attenuating inflammation and pyroptosis

Soy protein increases cognitive level in mice by modifying hippocampal nerve growth, oxidative stress, and intestinal microbiota

Activated astrocytes attenuate neocortical seizures in rodent models through driving Na+-K+-ATPase

CXCR4 knockout induces neuropathological changes in the MPTP-lesioned model of Parkinson's disease

A silk-based self-adaptive flexible opto-electro neural probe

A tannic acid doped hydrogel with small extracellular vesicles derived from mesenchymal stem cells promotes spinal cord repair by regulating reactive oxygen species microenvironment

Mechanobiological responses of astrocytes in optic nerve head due to biaxial stretch

Bu Shen Huo Xue decoction promotes functional recovery in spinal cord injury mice by improving the microenvironment to promote axonal regeneration

Stimulation by Exosomes from Hypoxia Preconditioned Human Umbilical Vein Endothelial Cells Facilitates Mesenchymal Stem Cells Angiogenic Function for Spinal Cord Repair

LiuweiDihuang improved cognitive functions in SAMP8 mice by inhibiting COX-2 expression and subsequent neuroinflammation

Inhibition of neuronal nitric oxide synthase protects against hippocampal neuronal injuries by increasing neuropeptide Y expression in temporal lobe epilepsy mice

Physical Exercise-Induced Astrocytic Neuroprotection and Cognitive Improvement Through Primary Cilia and Mitogen-Activated Protein Kinases Pathway in Rats With Chronic Cerebral Hypoperfusion

A reactive oxygen species-responsive hydrogel encapsulated with bone marrow derived stem cells promotes repair and regeneration of spinal cord injury

Hypoxia-stimulated mesenchymal stem cell-derived exosomes loaded by adhesive hydrogel for effective angiogenic treatment of spinal cord injury

Rapid and effective treatment of traumatic spinal cord injury using stem cell derived exosomes

Different changes in pre- and postsynaptic components in the hippocampal CA1 subfield after transient global cerebral ischemia

Tauroursodeoxycholic acid alleviates secondary injury in spinal cord injury mice by reducing oxidative stress, apoptosis, and inflammatory response

Protective effect of rapamycin in models of retinal degeneration

N-acetylserotonin alleviated the expression of interleukin-1β in retinal ischemia–reperfusion rats via the TLR4/NF-κB/NLRP3 pathway

Glial activation and inflammation in the NTS in a rat model after exposure to diesel exhaust particles

Olfactory ensheathing cell transplantation alters the expression of chondroitin sulfate proteoglycans and promotes axonal regeneration after spinal cord injury

Injectable silk sericin scaffolds with programmable shape-memory property and neuro-differentiation-promoting activity for individualized brain repair of severe ischemic stroke

Abnormal Glu/mGluR2/3/PI3K pathway in the hippocampal neurovascular unit leads to diabetes-related depression

Let-7f promotes the differentiation of neural stem cells in rats

Engineering Stem Cell Derived Biomimetic Vesicles for Versatility and Effective Targeted Delivery

Neuroinflammation changes with periodontal inflammation status during periodontitis in wild-type mice