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Product Info Summary
| SKU: | M00213-7 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246
SKU/Catalog Number
M00213-7
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (Catalog # M00213-7). Tested in IHC, WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00213-7)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM246
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the N-terminus of human GFAP
Cross-reactivity
This antibody reacts to human and mouse GFAP (Glial fibrillary acidic protein).
Reactive Species
M00213-7 is reactive to GFAP in Human, Mouse
Calculated molecular weight
49.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00213-7 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:200-1:500 dilution
WB: 1:1000-1:3000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of mouse brain tissue lysate, using Anti-GFAP RM246 at a 1:2500 dilution, showed GFAP (~50 kDa) expression in mouse brain.
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IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human brain tissue sections using Anti-GFAP RM246 at a 1:500 dilution.
Specific Publications For Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246 (M00213-7)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-GFAP Rabbit Monoclonal Antibody, Clone#RM246
Question
My question regards to test anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 on human dorsal motor nucleus of vagus nerve for research purposes, then I may be interested in using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-10
Answer
The products we sell, including anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-10
Question
Is there a BSA free version of anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 available?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-18
Question
Our lab want to know about using your anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 for chaperone mediated autophagy studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
D. Miller
Verified customer
Asked: 2019-09-10
Answer
I appreciate your inquiry. This M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 is validated on mouse brain, brain tissue, tissue lysate, human brain tissue. It is guaranteed to work for IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-10
Question
Is this M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 reactive to the isotypes of GFAP?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
The immunogen of M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 is A peptide corresponding to the N-terminus of human GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-26
Question
We purchased anti-GFAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I looked at the website and datasheets of our anti-GFAP Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-09
Question
Our team were well pleased with the WB result of your anti-GFAP Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
Based on literature, fetal brain cortex does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-03-07
Question
We are currently using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-21
Question
I have a question about product M00213-7, anti-GFAP Rabbit Monoclonal antibody, Clone#RM246. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Taylor
Verified customer
Asked: 2018-10-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-23
Question
Will anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 work for WB with dorsal motor nucleus of vagus nerve?
L. Rodriguez
Verified customer
Asked: 2018-10-11
Answer
According to the expression profile of dorsal motor nucleus of vagus nerve, GFAP is highly expressed in dorsal motor nucleus of vagus nerve. So, it is likely that anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 will work for WB with dorsal motor nucleus of vagus nerve.
Boster Scientific Support
Answered: 2018-10-11
Question
I was wanting to use your anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 for WB for human dorsal motor nucleus of vagus nerve on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human dorsal motor nucleus of vagus nerve identification?
Verified Customer
Verified customer
Asked: 2018-03-09
Answer
It shows on the product datasheet, M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human dorsal motor nucleus of vagus nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-03-09
Question
I have attached the WB image, lot number and protocol we used for dorsal motor nucleus of vagus nerve using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for dorsal motor nucleus of vagus nerve using anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7. Let me know if you need anything else.
C. Parker
Verified customer
Asked: 2018-02-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-22
Question
Is a blocking peptide available for product anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7)?
D. Anderson
Verified customer
Asked: 2017-09-26
Answer
We do provide the blocking peptide for product anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 (M00213-7). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-09-26
Question
Would M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
V. Krishna
Verified customer
Asked: 2015-09-03
Answer
As indicated on the product datasheet, M00213-7 anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-09-03
Question
We have been able to see staining in human dorsal motor nucleus of vagus nerve. Are there any suggestions? Is anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 supposed to stain dorsal motor nucleus of vagus nerve positively?
K. Anderson
Verified customer
Asked: 2014-10-06
Answer
From literature dorsal motor nucleus of vagus nerve does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2014-10-06
Question
I see that the anti-GFAP Rabbit Monoclonal antibody, Clone#RM246 M00213-7 works with WB, what is the protocol used to produce the result images on the product page?
H. Anderson
Verified customer
Asked: 2014-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2014-03-17
