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Product Info Summary
| SKU: | A02140-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-GIT1 Antibody
SKU/Catalog Number
A02140-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-GIT1 Antibody catalog # A02140-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GIT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02140-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human GIT1. AA range:561-610
Reactive Species
A02140-1 is reactive to GIT1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
84.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02140-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:20000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human spleen tissue. 1,primary Antibody was diluted at 1:200 (4° overnight). 2, Sodium citrate pH 6.0 was used for antigen retrieval (>98°C,20min). 3,Secondary antibody was diluted at 1:200
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Western Blot analysis of HepG2 cells using GIT1 Polyclonal Antibody
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Western blot analysis of the lysates from 293 cells using GIT1 antibody.
Specific Publications For Anti-GIT1 Antibody (A02140-1)
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Customer Q&As
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3 Customer Q&As for Anti-GIT1 Antibody
Question
I see that the anti-GIT1 antibody A02140-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-21
Question
Is a blocking peptide available for product anti-GIT1 antibody (A02140-1)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do provide the blocking peptide for product anti-GIT1 antibody (A02140-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-14
Question
We are currently using anti-GIT1 antibody A02140-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
S. Parker
Verified customer
Asked: 2015-11-09
Answer
The anti-GIT1 antibody (A02140-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-09
