Anti-Gli-3 Antibody

Product Name

Anti-Gli-3 Antibody

View all GLI3 Antibodies

SKU/Catalog Number

A01171

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Gli-3 Antibody (Catalog # A01171). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Gli-3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01171)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was produced from monospecific rabbit serum by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 30-60 of human Gli-3 protein.

Reactive Species

A01171 is reactive to GLI3 in Human

Observed Molecular Weight

42 kDa

Calculated molecular weight

169.9 kDa

Background of GLI3

Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS); an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190kDa and the other is a truncated isoform at ~80kDa.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01171 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:6,000 - 1:30,000
IHC: 0.5 mg/ml - 5 µg/ml
IF Microscopy: User optimized
WB: 1:500 - 1:2,000
This antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and western blot.  Specific conditions for reactivity should be optimized by the end user. Detection of Gli-3 by western blot may be enhanced if nuclear extracts are used instead of whole cell lysates as the expression/abundance of Gli-3 is likely to be low. Furthermore, Gli3 expression is likely to be developmentally regulated and induced, making it difficult to detect in whole tissue homogenates.

Validation Images & Assay Conditions

See full target information GLI3

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