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Product Info Summary
| SKU: | M00527 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Gli1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00527
BM4606 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Gli1 Rabbit Monoclonal Antibody catalog # M00527. Tested in WB application. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Gli1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00527)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
GCG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Gli1
Reactive Species
M00527 is reactive to GLI1 in Human
Observed Molecular Weight
160 kDa
Calculated molecular weight
117.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00527 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of Gli1 expression in A549 cell lysate.
Specific Publications For Anti-Gli1 Rabbit Monoclonal Antibody (M00527)
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Gli1 Rabbit Monoclonal Antibody
Question
I would like to test anti-Gli1 Rabbit Monoclonal antibody M00527 on human tibial nerve for research purposes, then I may be interested in using anti-Gli1 Rabbit Monoclonal antibody M00527 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
The products we sell, including anti-Gli1 Rabbit Monoclonal antibody M00527, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-22
Question
Does M00527 anti-Gli1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
You can see on the product datasheet, M00527 anti-Gli1 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-04
Question
We are currently using anti-Gli1 Rabbit Monoclonal antibody M00527 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The anti-Gli1 Rabbit Monoclonal antibody (M00527) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-23
Question
Is a blocking peptide available for product anti-Gli1 Rabbit Monoclonal antibody (M00527)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
We do provide the blocking peptide for product anti-Gli1 Rabbit Monoclonal antibody (M00527). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-13
Question
Would anti-Gli1 Rabbit Monoclonal antibody M00527 work for WB with tibial nerve?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
According to the expression profile of tibial nerve, GLI1 is highly expressed in tibial nerve. So, it is likely that anti-Gli1 Rabbit Monoclonal antibody M00527 will work for WB with tibial nerve.
Boster Scientific Support
Answered: 2019-08-26
Question
I was wanting to use your anti-Gli1 Rabbit Monoclonal antibody for WB for human tibial nerve on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human tibial nerve identification?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
As indicated on the product datasheet, M00527 anti-Gli1 Rabbit Monoclonal antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human tibial nerve in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-01
Question
My team were happy with the WB result of your anti-Gli1 Rabbit Monoclonal antibody. However we have been able to see positive staining in tibial nerve cytoplasm using this antibody. Is that expected? Could you tell me where is GLI1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
According to literature, tibial nerve does express GLI1. Generally GLI1 expresses in cytoplasm. Regarding which tissues have GLI1 expression, here are a few articles citing expression in various tissues:
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-06-07
Question
I see that the anti-Gli1 Rabbit Monoclonal antibody M00527 works with WB, what is the protocol used to produce the result images on the product page?
R. Parker
Verified customer
Asked: 2019-05-24
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-24
Question
See attached the WB image, lot number and protocol we used for tibial nerve using anti-Gli1 Rabbit Monoclonal antibody M00527. Please let me know if you require anything else.
K. Bhatt
Verified customer
Asked: 2019-01-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-21
Question
Would anti-Gli1 Rabbit Monoclonal antibody M00527 work on monkey WB with tibial nerve?
Verified Customer
Verified customer
Asked: 2019-01-03
Answer
Our lab technicians have not tested anti-Gli1 Rabbit Monoclonal antibody M00527 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Gli1 Rabbit Monoclonal antibody M00527 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey tibial nerve in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibial nerve using anti-Gli1 Rabbit Monoclonal antibody M00527. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-07-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-07-30
Question
you antibody using your anti-Gli1 Rabbit Monoclonal antibody for postaxial studies. Has this antibody been tested with western blotting on a549 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
Thanks for your inquiry. This M00527 anti-Gli1 Rabbit Monoclonal antibody is tested on a549 cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-07-12
Question
We have seen staining in human muscle. What should we do? Is anti-Gli1 Rabbit Monoclonal antibody supposed to stain muscle positively?
D. Jackson
Verified customer
Asked: 2018-01-01
Answer
From what I have seen in literature muscle does express GLI1. From what I have seen in Uniprot.org, GLI1 is expressed in tibial nerve, muscle, among other tissues. Regarding which tissues have GLI1 expression, here are a few articles citing expression in various tissues:
Muscle, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-01-01
Question
I have a question about product M00527, anti-Gli1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00527 anti-Gli1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-19
Question
Is this M00527 anti-Gli1 Rabbit Monoclonal antibody reactive to the isotypes of GLI1?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
The immunogen of M00527 anti-Gli1 Rabbit Monoclonal antibody is A synthesized peptide derived from human Gli1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-10
Question
Do you have a BSA free version of anti-Gli1 Rabbit Monoclonal antibody M00527 available?
D. Huang
Verified customer
Asked: 2013-06-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Gli1 Rabbit Monoclonal antibody M00527 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-06-17
