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Product Info Summary
| SKU: | M07909 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB, IHC-P-Leica |
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Product info
Product Name
Anti-MAP4K1 Antibody
SKU/Catalog Number
M07909
Size
50 µl
Description
Boster Bio Anti-MAP4K1 Antibody (Catalog # M07909). Tested in WB, IHC-P-Leica application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-MAP4K1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M07909)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This MAP4K1 antibody is generated from a rabbit immunized with a recombinant protein from human MAP4K1.
Reactive Species
M07909 is reactive to MAP4K1 in Human
Calculated molecular weight
91.3 kDa
Background of MAP4K1
Serine/threonine-protein kinase, which may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway. May play a role in hematopoietic lineage decisions and growth regulation. Able to autophosphorylate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M07909 is guaranteed for WB, IHC-P-Leica Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P-Leica: 1:500
Validation Images & Assay Conditions
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Anti-MAP4K1 Antibody at 1:2000 dilution + Raji whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 91 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-MAP4K1 Antibody at 1:2000 dilution
Lane 1: Daudi whole cell lysate
Lane 2: Raji whole cell lysate
Lane 3: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 91 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human epityphlon tissue using M07909 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue using M07909 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Specific Publications For Anti-MAP4K1 Antibody (M07909)
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