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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-DDX3/DDX3X Antibody Picoband™
Boster Bio Anti-DDX3/DDX3X Antibody Picoband™ catalog # A00751-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX3/DDX3X Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00751-1)
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human DDX3 (2-28aa SHVAVENALGLDQQFAGLDLNSSDNQS), identical to the related mouse sequence.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins.
A00751-1 is reactive to DDX3X in Human, Mouse, Rat
A00751-1 is guaranteed for WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of DDX3 using anti-DDX3 antibody (A00751-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat brain tissue lysate,
Lane 2: mouse brain tissue lysate,
Lane 3: human MCF-7 whole Cell lysate,
Lane 4: human COLO-320 whole Cell lysate,
Lane 5: human A549 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DDX3 antigen affinity purified polyclonal antibody (Catalog # A00751-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DDX3 at approximately 73KD. The expected band size for DDX3 is at 73KD.
Gene/Protein Information For DDX3X (Source: Uniprot.Org, NCBI)
ATP-dependent RNA helicase DDX3X
DEAD box helicase family
DBXCAP-Rf; DDX14; DDX3DEAD/H box-3; DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked; DEAD box protein 3, X-chromosomal; DEAD box, X isoform; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3; EC 3.6.1; EC 188.8.131.52; helicase like protein 2; Helicase-like protein 2; HLP2ATP-dependent RNA helicase DDX3X DDX3X CAP-Rf, DBX, DDX14, DDX3, HLP2, MRX102, MRXSSB DEAD-box helicase 3 X-linked ATP-dependent RNA helicase DDX3X|DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked|DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked|DEAD box protein 3, X-chromosomal|DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3|DEAD/H box-3|helicase-like protein 2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on DDX3X, check out the DDX3X Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for DDX3X: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for A00751-1
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