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Product Info Summary
| SKU: | M00928 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2]
SKU/Catalog Number
M00928
Size
100 µl
Description
Boster Bio GSS (Glutathione Synthetase) mouse monoclonal antibody, clone OTI2F2 (formerly 2F2). Catalog# M00928. Tested in FC, IF, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00928)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F2
Isotype
IgG1
Immunogen
Full length human recombinant protein of human GSS (NP_000169) produced in HEK293T cell.
Reactive Species
M00928 is reactive to GSS in Dog, Human, Mouse, Rat
Calculated molecular weight
52.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00928 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IF 1:100
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells transfected with either GSS (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GSS antibody (M00928)
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Anti-GSS mouse monoclonal antibody (M00928) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GSS.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GSS (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GSS.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-GSS monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:500).
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and GSS-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-GSS monoclonal antibody M00928
Specific Publications For Anti-Glutathione Synthetase (GSS) Mouse Monoclonal Antibody [Clone ID: OTI2F2] (M00928)
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