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Product Info Summary
| SKU: | A12727 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-LRFN5 Antibody
SKU/Catalog Number
A12727
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-LRFN5 Antibody (Catalog # A12727). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
LRFN5 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-LRFN5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12727)
Host
Rabbit
Contents
LRFN5 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
LRFN5 antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human LRFN5. The immunogen is located within amino acids 650 - 700 of LRFN5.
Cross-reactivity
At least two isoforms of LRFN5 are known to exist. This antibody is predicted to not cross-react with other members of the LRFN family.
Reactive Species
A12727 is reactive to LRFN5 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
79.4 kDa
Background of LRFN5
LRFN5 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the Lrfn proteins play a role in the developing and/or mature vertebrate nervous system.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A12727 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
LRFN5 antibody can be used for detection of LRFN5 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of LRFN5 in EL4 cell lysate with LRFN5 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of LRFN5 in mouse brain tissue with LRFN5 antibody at 5 μg/mL.
Specific Publications For Anti-LRFN5 Antibody (A12727)
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