Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody

Product Name

Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody

View all GYS1 Antibodies

SKU/Catalog Number

P03512

Size

200ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody (Catalog # P03512). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store Phospho GYS1 antibody at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-Phospho-Glycogen Synthase 1 S641 GYS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03512)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Human Muscle Glycogen Synthase phospho peptide corresponding to pS641 region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

Reactive Species

P03512 is reactive to GYS1 in Human, Mouse

Calculated molecular weight

83.8 kDa

Background of GYS1

Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

P03512 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
IHC: 1:1,000 - 1:5,000
WB: 1:500 - 1:3,000
This phospho specific polyclonal antibody was tested by immunohistochemistry, immunoblotting, and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of the immunizing peptide and minimally reactive with the non-phosphorylated form of the immunizing peptide. Immunoblotting will detect human and mouse muscle glycogen synthase. Although not tested, this antibody is likely functional in immunoprecipitation.

Validation Images & Assay Conditions

See full target information GYS1

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