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Product Info Summary
| SKU: | M00339 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody
SKU/Catalog Number
M00339
BM5254 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody catalog # M00339. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00339)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACDE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome P450 3A4 Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Reactive Species
M00339 is reactive to CYP3A4 in Human
Observed Molecular Weight
50 kDa
Calculated molecular weight
57.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00339 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
IP 1:50
Positive Control
WB: Human fetal liver
Validation Images & Assay Conditions
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Western blot analysis of Cytochrome P450 3A4 expression in Human fetal liver lysate.
Specific Publications For Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody (M00339)
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Customer Q&As
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5 Customer Q&As for Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody
Question
Does M00339 cross-react with other CYP3A?
Verified customer
Asked: 2020-10-27
Answer
We have not experimentally validated cross-reactivity with other CYP3A using the Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody (M00339). The immunogen sequence of M00339 is LKVESRDGTVSGA.
Boster Scientific Support
Answered: 2020-10-30
Question
I have a question about product M00339, anti-Cytochrome P450 3A4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00339 anti-Cytochrome P450 3A4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-19
Question
Is this M00339 anti-Cytochrome P450 3A4 Monoclonal antibody reactive to the isotypes of CYP3A4?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The immunogen of M00339 anti-Cytochrome P450 3A4 Monoclonal antibody is A synthesized peptide derived from human Cytochrome P450 3A4 Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-12
Question
Does anti-Cytochrome P450 3A4 Monoclonal antibody M00339 work for IP with liver?
C. Lewis
Verified customer
Asked: 2017-06-27
Answer
According to the expression profile of liver, CYP3A4 is highly expressed in liver. So, it is likely that anti-Cytochrome P450 3A4 Monoclonal antibody M00339 will work for IP with liver.
Boster Scientific Support
Answered: 2017-06-27
Question
Would M00339 anti-Cytochrome P450 3A4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Krishna
Verified customer
Asked: 2015-06-01
Answer
It shows on the product datasheet, M00339 anti-Cytochrome P450 3A4 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2015-06-01
