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- Table of Contents
CYP3A4 antibody (M00339) performs well in IP, IHC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Western blot analysis of Cytochrome P450 3A4 expression in Human fetal liver lysate.
Designing a Western blot or IHC for CYP3A4? The guides cover the expected band size and staining pattern, antibodies backed by real images, the controls to run, and protocols from published papers.
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Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody
CYP3A4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | IP, IHC, WB |
Tested Applications
CYP3A4 antibody was positively detected by WB in:
Human fetal liver
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
WB
1:1000-1:5000
IHC
1:50-1:200
IP
1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody
View all CYP3A4 Antibodies
View all CYP3A4 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ACDE-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytochrome P450 3A4 Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. The enzyme also hydroxylates etoposide.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
50 kDa
Calculated Molecular Weight
57.3 kDa
Cite This Product
Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00339)
Protocols
Western Blot protocol for CYP3A4 antibody
Review
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Product Promise
We promise Anti-Cytochrome P450 3A4 CYP3A4 Monoclonal Antibody performs excellently in relevant applications for IP, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00339
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 23, 2026