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Product Info Summary
| SKU: | M02184-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Glycophorin A (CD235a) GYPA Monoclonal Antibody
SKU/Catalog Number
M02184-1
BM5354 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Glycophorin A (CD235a) GYPA Monoclonal Antibody catalog # M02184-1. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glycophorin A (CD235a) GYPA Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02184-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ADOG-7
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Glycophorin A (CD235a).
Reactive Species
M02184-1 is reactive to GYPA in Human
Observed Molecular Weight
38 kDa
Calculated molecular weight
16.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02184-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
IHC:human colon cancer tissue, human lung cancer tissue
Validation Images & Assay Conditions
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Western blot analysis of Glycophorin A (CD235a) expression in human fetal liver lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1).
Glycophorin A/GYPA was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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IHC analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1).
Glycophorin A/GYPA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with a dilution of 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
IHC analysis of Glycophorin A/GYPA using anti-Glycophorin A/GYPA antibody (M02184-1).
Glycophorin A/GYPA was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:100 rabbit anti-Glycophorin A/GYPA Antibody (M02184-1) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Specific Publications For Anti-Glycophorin A (CD235a) GYPA Monoclonal Antibody (M02184-1)
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