Click image to see more details
Product Info Summary
| SKU: | PB9028 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-I-309/CCL1 Antibody Picoband®
SKU/Catalog Number
PB9028
PB0052 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-I-309/CCL1 Antibody Picoband® catalog # PB9028. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-I-309/CCL1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9028)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human I-309 recombinant protein (Position: K24-K96). Human I-309 shares 38% amino acid (aa) sequence identity with mouse I-309.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9028 is reactive to CCL1 in Human
Observed Molecular Weight
11 kDa
Calculated molecular weight
11.0 kDa
Background of CCL1
CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9028 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, -
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: U87 Whole Cell, MCF-7 Whole Cell, COLO320 Whole Cell
Validation Images & Assay Conditions
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Western blot analysis of I-309 using anti-I-309 antibody (PB9028).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U87 Whole Cell Lysate,
Lane 2: MCF-7 Whole Cell Lysate,
Lane 3: COLO320 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-I-309 antigen affinity purified polyclonal antibody (Catalog # PB9028) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for I-309 at approximately 11KD. The expected band size for I-309 is at 11KD.
Specific Publications For Anti-I-309/CCL1 Antibody Picoband® (PB9028)
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Customer Q&As
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6 Customer Q&As for Anti-I-309/CCL1 Antibody Picoband®
Question
We are currently using anti-I-309/CCL1 antibody PB9028 for human tissue, and we are content with the ELISA results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
P. Baker
Verified customer
Asked: 2020-02-10
Answer
The anti-I-309/CCL1 antibody (PB9028) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-10
Question
Do you have a BSA free version of anti-I-309/CCL1 antibody PB9028 available?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-I-309/CCL1 antibody PB9028 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-08
Question
Here is the WB image, lot number and protocol we used for brain using anti-I-309/CCL1 antibody PB9028. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-08
Question
I see that the anti-I-309/CCL1 antibody PB9028 works with ELISA, what is the protocol used to produce the result images on the product page?
J. Baker
Verified customer
Asked: 2019-05-30
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-05-30
Question
I have a question about product PB9028, anti-I-309/CCL1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Carter
Verified customer
Asked: 2018-11-26
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9028 anti-I-309/CCL1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-26
Question
I was wanting to use your anti-I-309/CCL1 antibody for ELISA for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
You can see on the product datasheet, PB9028 anti-I-309/CCL1 antibody has been validated for ELISA, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-19
