Anti-I-309/CCL1 IGF1R Antibody Picoband™

Product Name

Anti-I-309/CCL1 IGF1R Antibody Picoband™

View all IGF-I R/IGF1R Antibodies

SKU/Catalog Number

PB9028

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-I-309/CCL1 IGF1R Antibody Picoband™ catalog # PB9028. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-I-309/CCL1 IGF1R Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9028)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human I-309 recombinant protein (Position: K24-K96). Human I-309 shares 38% amino acid (aa) sequence identity with mouse I-309.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9028 is reactive to IGF1R in Human

Applications

PB9028 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

11 kDa

Calculated molecular weight

154.793kDa

Background of IGF-I R/IGF1R

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, -
ELISA , 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of I-309 using anti-I-309 antibody (PB9028).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: U87 Whole Cell Lysate,
Lane 2: MCF-7 Whole Cell Lysate,
Lane 3: COLO320 Whole Cell Lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-I-309 antigen affinity purified polyclonal antibody (Catalog # PB9028) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for I-309 at approximately 11KD. The expected band size for I-309 is at 11KD.

Gene/Protein Information For IGF1R (Source: Uniprot.org, NCBI)

Alternative Names

CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2

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