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- Table of Contents
ANO1 antibody (PA2290) performs well in WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Anti-TMEM16A antibody, PA2290, All Western blotting
All lanes: Anti-ANO1(PA2290) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Predicted bind size: 114KD
Observed bind size: 114KD
All lanes: Anti-ANO1(PA2290) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Predicted bind size: 114KD
Observed bind size: 114KD
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Anti-TMEM16A/ANO1 Antibody Picoband®
ANO1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | WB |
Tested Applications
ANO1 antibody was positively detected by WB in:
Rat Liver Tissue, Rat Skeletal Muscle Tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Rat, Human, Mouse
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
Rat
Tested
Human
Predicted
Monkey
Predicted
Mouse
Predicted
Bovine
Predicted
Sheep
Predicted
Dog
Predicted
Product Information
Product Name
Anti-TMEM16A/ANO1 Antibody Picoband®
View all ANO1 Antibodies
View all ANO1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A, different from the related mouse and rat sequences by one amino acid.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
114 kDa
Calculated Molecular Weight
114.1 kDa
Background of ANO1
Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2) or tumor-amplified and overexpressed sequence 2 (TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.
Cite This Product
Anti-TMEM16A/ANO1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2290)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Mouse
94%
High Homology
Rat
94%
High Homology
Cow
94%
High Homology
Sheep
94%
High Homology
Dog
94%
High Homology
Zebrafish
71%
Moderate Homology
Drosophila
12%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ANO1 antibody
Publications
Application:
Species:
ANO1, CaV1.2, and IP3R form a localized unit of EC-coupling in mouse pulmonary arterial smooth muscle
Published: 2023-09-13
Journal: JOURNAL OF GENERAL PHYSIOLOGY
Application: IF
Impact Factor: 3.80
Species: Mouse
PMID: 37702787
Intestinal TMEM16A function as a luminal chloride channel
Published: 2020-04-18
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.97
Species: Mouse
Review
Have you used Anti-TMEM16A/ANO1 Antibody Picoband®?
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Submit A Review1 Review For Anti-TMEM16A/ANO1 Antibody Picoband®
The staining is clear, highly specific, with almost no non-specific bands.
| SKU | PA2290 |
|---|---|
| Application | Western Blot |
| Sample | Protein extracts from LA795 and 16HBE cells. |
| Sample Processing Description | After collecting the cells, lyse them with RIPA buffer containing protease inhibitors, quantify the protein using the BCA assay, and add loading buffer proportionally. Denature by heating at 98 °C. Load the samples onto SDS-PAGE, with 30 μg of total protein per lane. |
| Primary Antibody | Anti-TMEM16A/ANO1 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Blocking Agent | 5% non-fat milk |
| Secondary Antibody | HRP-conjugated goat anti-rabbit IgG |
| Secondary Incubation | Incubate at room temperature for 1 hour |
| Detection | Substrate: ECL substrate, Imaging system: Tanon |
| Results Summary | The TMEM16A antibody we previously used was never ideal; despite long-term optimization, it was difficult to obtain clear bands. This time, by using BOSTER’s polyclonal antibody, we achieved much better experimental results. The bands were successfully exposed in essentially one attempt, with clear signals and no non-specific bands. We are very satisfied with Boster’s antibody. |
Shi Sai, Hebei University of Technology
Verified customer
Submitted 2025-10-27
Product Promise
We promise Anti-TMEM16A/ANO1 Antibody Picoband® performs excellently in relevant applications for WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA2290
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Sep 25, 2026