Anti-TMEM16A/ANO1 Antibody

Product Name

Anti-TMEM16A/ANO1 Antibody

View all DOG1/TMEM16A Antibodies

SKU/Catalog Number

PA2290

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-TMEM16A/ANO1 Antibody catalog # PA2290. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-TMEM16A/ANO1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2290)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human TMEM16A, different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2290 is reactive to ANO1 in Human, Mouse, Rat

Applications

PA2290 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

114 kDa

Calculated molecular weight

114.078kDa

Background of DOG1/TMEM16A

Anoctamin-1 (ANO1), also known as oral cancer overexpressed 2 (ORAOV2) or tumor-amplified and overexpressed sequence 2 (TMEM16A), is a protein that in humans is encoded by the ANO1 gene. This gene belongs to a family of membrane proteins containing 8 transmembrane segments, and it is mapped to 11q13.3. ANO1 is a candidate calcium-activated chloride channel that mediates receptor-activated chloride currents in diverse physiologic processes, and it is though to be responsible for a voltage-sensitive calcium-activated chloride current. Its overexpression was reported in esophageal squamous cell carcinoma and breast cancer progression Crofelemer, an antidiarrhoeal, inhibits this channel. ANO1 has eight transmembrane domains, its pore is large and non-selective, allowing other negatively charged species to permeate.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Validation Images & Assay Conditions

Click image to see more details

Anti-TMEM16A antibody, PA2290, All Western blotting
All lanes: Anti-ANO1(PA2290) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 40ug
Lane 2: Rat Skeletal Muscle Tissue Lysate at 40ug
Predicted bind size: 114KD
Observed bind size: 114KD

Gene/Protein Information For ANO1 (Source: Uniprot.org, NCBI)

Alternative Names

ANO1; anoctamin 1; DOG1; ORAOV2; TAOS2; TMEM16A ANO1 DOG1, ORAOV2, TAOS2, TMEM16A anoctamin 1 anoctamin-1|Ca2+-activated Cl- channel|anoctamin 1, calcium activated chloride channel|calcium activated chloride channel|discovered on gastrointestinal stromal tumors protein 1|oral cancer overexpressed 2|transmembrane protein 16A (eight membrane-spanning domains)|tumor-amplified and overexpressed sequence 2

For more info on ANO1, check out the ANO1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ANO1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis