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Product Info Summary
| SKU: | A10520 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-TAS2R14/T2R14 Antibody
SKU/Catalog Number
A10520
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TAS2R14/T2R14 Antibody (Catalog# A10520). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TAS2R14/T2R14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10520)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TAS2R14. Immunogen sequence location: 229 - 278
Reactive Species
A10520 is reactive to TAS2R14 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
36.2 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10520 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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Western blot analysis of the lysates from COLO205 cells using TAS2R14 antibody.
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Immunofluorescence analysis of MCF7 cells, using TAS2R14 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from MCF-7 cells, using TAS2R14 Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TAS2R14/T2R14 Antibody (A10520)
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Customer Q&As
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4 Customer Q&As for Anti-TAS2R14/T2R14 Antibody
Question
We are currently using anti-TAS2R14/T2R14 antibody A10520 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
The anti-TAS2R14/T2R14 antibody (A10520) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-10
Question
Will anti-TAS2R14/T2R14 antibody A10520 work for ELISA with plasma?
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
According to the expression profile of plasma, TAS2R14 is highly expressed in plasma. So, it is likely that anti-TAS2R14/T2R14 antibody A10520 will work for ELISA with plasma.
Boster Scientific Support
Answered: 2019-01-16
Question
Would anti-TAS2R14/T2R14 antibody A10520 work on zebrafish IF with plasma?
Z. Li
Verified customer
Asked: 2017-01-09
Answer
Our lab technicians have not validated anti-TAS2R14/T2R14 antibody A10520 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TAS2R14/T2R14 antibody A10520 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish plasma in IF, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-01-09
Question
My question regarding product A10520, anti-TAS2R14/T2R14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Parker
Verified customer
Asked: 2014-10-22
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10520 anti-TAS2R14/T2R14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-22
