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- Table of Contents
GPX1 antibody (M01019-2) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 2 Publications
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Flow Cytometry analysis of U87 cells using anti- GPX1 antibody (M01019-2).
Overlay histogram showing U87 cells stained with M01019-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (M01019-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U87 cells stained with M01019-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-GPX1 Antibody (M01019-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GPX1 Antibody Picoband® (monoclonal, 8B10)
GPX1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
GPX1 antibody was positively detected by WB in:
human placenta tissue, human U-87 whole cell, human THP-1 whole cell, human U-937 whole cell, human HepG2 whole cell, rat brain tissue, rat thymus tissue, rat lung tissue, rat liver tissue, mouse brain tissue, mouse thymus tissue, mouse lung tissue, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GPX1 antibody was positively detected by IHC in:
human lung cancer tissue, human mammary cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GPX1 antibody was positively detected by IF in:
human colon cancer tissue
GPX1 antibody was positively detected by FC in:
U87 cell, U251 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunocytochemistry/Immunofluorescence
5μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
Human
Tested
Tested
Recommend
Tested
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GPX1 Antibody Picoband® (monoclonal, 8B10)
View all GPX1 Antibodies
View all GPX1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
8B10
Isotype
Mouse IgG2b
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human GPX1, different from the related mouse sequence by six amino acids and from the related rat sequence by five amino acids.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
22 kDa
Calculated Molecular Weight
22.1 kDa
Background of GPX1
Glutathione peroxidase 1, also known as, GPX-1 is an enzyme that in humans is encoded by the GPX1 gene. It is mapped to 3p21.31. This gene encodes a member of the glutathione peroxidase family, consisting of eight known glutathione peroxidases (Gpx1-8) in humans. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. It has been reported that the protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell growth and increases resistance to toxic challenges. GPX1 is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.
Cite This Product
Anti-GPX1 Antibody Picoband® (monoclonal, 8B10) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01019-2)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
100%
High Homology
Dog
90%
High Homology
Rabbit
87%
Moderate Homology
Cow
87%
Moderate Homology
Sheep
87%
Moderate Homology
Rat
84%
Moderate Homology
Mouse
81%
Moderate Homology
Zebrafish
71%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GPX1 antibody
Immunohistochemistry (IHC) protocol for GPX1 antibody
Immunofluorescence (IF) protocol for GPX1 antibody
Flow Cytometry (FC) protocol for GPX1 antibody
Publications
Application:
Species:
Exercise Training Stimulates the Release of Glutathione Peroxidase 1 (GPX1)‐Enriched Extracellular Vesicles That Promote Angiogenesis
Published: 2026-06-18
Journal: FASEB JOURNAL
Application: WB
Impact Factor: 4.30
Species: Mouse
PMID: 42313915
Injectable hydrogel with selenium nanoparticles delivery for sustained glutathione peroxidase activation and enhanced osteoarthritis therapeutics
Published: 2023-11-11
Journal: Materials Today Bio
Application: IHC
Impact Factor: 8.20
Species: Rat
PMID: 38024839
Review
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Product Promise
We promise Anti-GPX1 Antibody Picoband® (monoclonal, 8B10) performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
M01019-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 24, 2026