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Product Info Summary
| SKU: | PA1899 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband®
SKU/Catalog Number
PA1899
BA3836-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Superoxide Dismutase 3/SOD3 Antibody catalog # PA1899. Tested in IHC, IHC-F, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1899)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SOD3.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1899 is reactive to SOD3 in Human
Observed Molecular Weight
60 kDa
Calculated molecular weight
25.9 kDa
Background of SOD3
SOD3 (SUPEROXIDE DISMUTASE 3) also called SUPEROXIDE DISMUTASE, EXTRACELLULAR, EC-SOD, and Cu-Zn, is an enzyme that in humans is encoded by the SOD3 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Hendrickson et al. (1990) mapped the SOD3 gene to 4pter-q21 by a study of somatic cell hybrids. Stern et al. (2003) narrowed the assignment to 4p15.3-p15.1 by somatic cell and radiation hybrid analysis, linkage mapping, and FISH. The product of this gene is though to protect the brain, lungs, and other tissues from oxidative stress. The protein is secreted into the extracellular space and forms a glycosylated homotetramer that is anchored to the extracellular matrix (ECM) and cell surfaces through an interaction with heparan sulfate proteoglycan and collagen. A fraction of the protein is cleaved near the C-terminus before secretion to generate circulating tetramers that do not interact with the ECM.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1899 is guaranteed for IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human,
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human,
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, A549 Cell, MM231 Cell, MCF-7 Cell
IHC: human mammary cancer tissue
IHC-F: Human Placenta Tissue
ICC: HELA cell
Validation Images & Assay Conditions
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Anti-SOD3 antibody, PA1899, Western blotting
Lane 1: Human Placenta Tissue Lysate
Lane 2: A549 Cell Lysate
Lane 3: MM231 Cell Lysate
Lane 4: MCF-7 Cell Lysate
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Anti-SOD3 antibody, PA1899, IHC(F)
IHC(F): Human Placenta Tissue
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IHC analysis of SOD3 using anti-SOD3 antibody (PA1899).
SOD3 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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ICC analysis of SOD3 using anti-SOD3 antibody (PA1899).
SOD3 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1μg/ml rabbit anti-SOD3 Antibody (PA1899) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Specific Publications For Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband® (PA1899)
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6 Customer Q&As for Anti-Superoxide Dismutase 3/SOD3 Antibody Picoband®
Question
Is this PA1899 anti-Superoxide Dismutase 3/SOD3 antibody reactive to the isotypes of SOD3?
Verified Customer
Verified customer
Asked: 2020-04-13
Answer
The immunogen of PA1899 anti-Superoxide Dismutase 3/SOD3 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human SOD3 (41-56aa KVTEIWQEVMQRRDDD). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-04-13
Question
Please see the WB image, lot number and protocol we used for blood using anti-Superoxide Dismutase 3/SOD3 antibody PA1899. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-17
Question
Is there a BSA free version of anti-Superoxide Dismutase 3/SOD3 antibody PA1899 available?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Superoxide Dismutase 3/SOD3 antibody PA1899 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-14
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-Superoxide Dismutase 3/SOD3 antibody PA1899. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-11
Question
Is a blocking peptide available for product anti-Superoxide Dismutase 3/SOD3 antibody (PA1899)?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
We do provide the blocking peptide for product anti-Superoxide Dismutase 3/SOD3 antibody (PA1899). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-07-30
Question
Does anti-Superoxide Dismutase 3/SOD3 antibody PA1899 work for ICC with blood?
J. Williams
Verified customer
Asked: 2015-08-20
Answer
According to the expression profile of blood, SOD3 is highly expressed in blood. So, it is likely that anti-Superoxide Dismutase 3/SOD3 antibody PA1899 will work for ICC with blood.
Boster Scientific Support
Answered: 2015-08-20
