Human Granulysin PicoKine® Quick ELISA Kit

Granulysin ELISA kit, 90-minute one-step protocol

Human Granulysin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GNLY in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK1280
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Assay Time: 90 Minutes

Introduction

Boster's Picokine™ Quick ELISA kits features the high sensitivity of Picokine™ ELISA kits and the significantly reduced assay run time. Now you can run the entire ELISA assay in less than 1.5 hours.

Compared to regular Picokine ELISA kits, the following sections in the datasheet are different:
1. kit components;
2. preparation before experiment;
3. assay protocol

Product Name

Human Granulysin PicoKine® Quick ELISA Kit

See all Granulysin products

SKU/Catalog Number

FEK1280

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Granulysin PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human GNLY in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Granulysin PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1280)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-L145

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK1280 is reactive to GNLY in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

FEK1280 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Granulysin

Granulysin is a substance released by cytotoxic T cells (CD8) when they are attached to infected body cells. The product of this gene is a member of the saposin-like protein (SAPLIP) family. It is mapped to 2p11.2. Granulysin functions to create holes in the target cell membrane and destroy it. It is able to induce apoptosis in target cells and also has antimicrobial action. This gene is expressed in cytolytic granules with perforin, a pore forming protein, and granzymes that are also involved in cytolysis. In addition to it, Granulysin is broadly antimicrobial, killing microbes that cause, for example, tuberculosis and malaria, and can destroy some tumors. A series of peptides generated from the amino acid sequence of Granulysin are potential antibiotics. It has been found that secretory Granulysin is a key molecule responsible for the disseminated keratinocyte death in SJS/TEN.

Kit Components

Catalog number Description Quantity
FEK1280-CAP 96-well plate precoated with anti-Human Granulysin antibody 1
FEK1280-ST lyophilized recombinant Human Granulysin standard 10 ng/tube
FEK1280-DA Biotinylated anti-Human Granulysin detection antibody (50x) 100 ul
- Avidin-Biotin-Peroxidase Complex (30x) 400 µl
AR1106-1 Sample diluent 30 ml
AR1106-2 Antibody diluent 12 ml
- Avidin-Biotin-Peroxidase Diluent 12 ml
AR1106-5 Wash Buffer (25x) 20 ml
AR1104 Color Developing Reagent (TMB) 10 ml
AR1105 Stop Solution 10 ml
PLA-SEA Plate Sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0120.0900.1730.3370.6551.1031.8902.458

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5725891156266859
Standard deviation4.1612.6460.124.1413.362.7
CV (%)7.3%4.9%6.6%7.4%5%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Granulysin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 157495550523.346.4%
Sample 225824022522223614.286%
Sample 391177481985183849.885.9%
*number of samples for each test n=16.

Gene/Protein Information For GNLY (Source: Uniprot.Org, NCBI)

Uniprot ID

P22749

Gene ID

10578

Gene Name

GNLY

Full Name

Granulysin

Weight

16.374kDa

Alternative Names

D2S69Elymphocyte-activation gene 2; GNLY; Granulysin; LAG2; LAG-2; Lymphokine LAG-2; Lymphokine; NKG5; NKG5519; Protein NKG5; TLA519; TLA519T-cell activation protein 519; T-lymphocyte activation gene 519 GNLY D2S69E, LAG-2, LAG2, NKG5, TLA519 granulysin granulysin|T-cell activation protein 519|T-lymphocyte activation gene 519|lymphocyte-activation gene 2|lymphokine LAG-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GNLY, check out the GNLY Infographic

GNLY infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GNLY: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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1 Customer Q&As for Human Granulysin PicoKine® Quick ELISA Kit

Question

Is FEK1280 suitable for detection of granulysin in human placental protein samples (tissue homogenates)?

Verified customer

Asked: 2021-02-16

Answer

Our lab hasn't worked on Human placental protein samples (tissue homogenates) using the Human Granulysin PicoKine™ Quick ELISA Kit (FEK1280). Endogenous biotin in tissue homogenates might introduce false positive signals. We recommend the customer to run a pilot test as described below to confirm the effect of endogenous biotin first. Add tissue homogenates into the wells and then add ABC and TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, proceed and work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2021-02-19

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