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Product Info Summary
| SKU: | PA1834 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Peroxiredoxin 2/PRDX2 Antibody Picoband®
SKU/Catalog Number
PA1834
BA3784 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Peroxiredoxin 2/PRDX2 Antibody catalog # PA1834. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Peroxiredoxin 2/PRDX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1834)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 2, identical to the related mouse and rat sequences.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1834 is reactive to PRDX2 in Human, Mouse, Rat
Observed Molecular Weight
48-52 kDa
Calculated molecular weight
21.9 kDa
Background of PRDX2
PRDX2 (peroxiredoxin 2) also known as NKEFB, PRP, PRX2, PRXII or TPX1, is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8 (+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The Prdx2 -/- mice were healthy in appearance and fertile. Choi et al. (2005) demonstrated that PRDX2 is a negative regulator of PDGF signaling. Prx II deficiency results in increased production of peroxide, enhanced activation of PDGF receptor and phospholipase C-gamma-1, and subsequently increased cell proliferation and migration in response to PDGF. PRX2 accounted for about half of the thioredoxin activity in parasite extracts, and PRX2 expression was increased in the presence of chloroquine, regardless of P. falciparum strain susceptibility to the drug.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1834 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Kidney Tissue, HELA Cell, JURKAT Cell, 293T Cell, A549 Cell,
Validation Images & Assay Conditions
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Anti-Peroxiredoxin 2 antibody, PA1834, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
Lane 3: HELA Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: 293T Cell Lysate
Lane 6: A549 Cell Lysate
Specific Publications For Anti-Peroxiredoxin 2/PRDX2 Antibody Picoband® (PA1834)
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Customer Q&As
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7 Customer Q&As for Anti-Peroxiredoxin 2/PRDX2 Antibody Picoband®
Question
Do you have a BSA free version of anti-Peroxiredoxin 2/PRDX2 antibody PA1834 available?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Peroxiredoxin 2/PRDX2 antibody PA1834 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-29
Question
Does anti-Peroxiredoxin 2/PRDX2 antibody PA1834 work on goat WB with liver?
M. Williams
Verified customer
Asked: 2019-10-11
Answer
Our lab technicians have not validated anti-Peroxiredoxin 2/PRDX2 antibody PA1834 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Peroxiredoxin 2/PRDX2 antibody PA1834 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-11
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellum using anti-Peroxiredoxin 2/PRDX2 antibody PA1834. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-04
Question
I am looking for to test anti-Peroxiredoxin 2/PRDX2 antibody PA1834 on human cerebellum for research purposes, then I may be interested in using anti-Peroxiredoxin 2/PRDX2 antibody PA1834 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The products we sell, including anti-Peroxiredoxin 2/PRDX2 antibody PA1834, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-31
Question
I was wanting to use your anti-Peroxiredoxin 2/PRDX2 antibody for WB for human cerebellum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cerebellum identification?
Verified Customer
Verified customer
Asked: 2019-02-05
Answer
As indicated on the product datasheet, PA1834 anti-Peroxiredoxin 2/PRDX2 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellum in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-05
Question
We are currently using anti-Peroxiredoxin 2/PRDX2 antibody PA1834 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-29
Answer
The anti-Peroxiredoxin 2/PRDX2 antibody (PA1834) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-29
Question
Is this PA1834 anti-Peroxiredoxin 2/PRDX2 antibody reactive to the isotypes of PRDX2?
E. Rodriguez
Verified customer
Asked: 2016-09-27
Answer
The immunogen of PA1834 anti-Peroxiredoxin 2/PRDX2 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Peroxiredoxin 2(181-198aa DTIKPNVDDSKEYFSKHN), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-09-27
