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Product Info Summary
| SKU: | M04115 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IP, WB |
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Product info
Product Name
Anti-GRAP2 Purified Monoclonal Antibody
SKU/Catalog Number
M04115
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-GRAP2 Purified Monoclonal Antibody (Catalog# M04115). Tested in Flow Cytometry, IP, WB application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-GRAP2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04115)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
UW40
Isotype
Mouse IgG2a
Immunogen
GST-fusion human GRAP2/GADS protein. The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.
Cross-reactivity
This antibody does not cross-react with Thy-1.1 alloantigen.
Reactive Species
M04115 is reactive to GRAP2 in Human
Observed Molecular Weight
42 kDa
Calculated molecular weight
37.9 kDa
Background of GRAP2
GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04115 is guaranteed for Flow Cytometry, IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 2-6 μg/ml. Intracellular staining.
Validation Images & Assay Conditions
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Separation of human GRAP2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-GRAP2 (UW40) purified antibody (concentration in sample 5 µg/ml, GAM APC).
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Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-GRAP2 (UW40) purified antibody (concentration in sample 5 µg/ml, GAM APC).
Specific Publications For Anti-GRAP2 Purified Monoclonal Antibody (M04115)
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