|Validated Species:||Mouse, Rat|
Data & Images
|Product Name||Anti-GHR/Growth Hormone R Antibody|
|Description||Rabbit IgG polyclonal antibody for Growth hormone receptor(GHR) detection. Tested with WB in Mouse;Rat.|
|Cite This Product||Anti-GHR/Growth Hormone R Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1726)|
|Replacement Item||This antibody may replace the following items: sc-57161|sc-20747|sc-71146|sc-10355|sc-10354|sc-10351|sc-69882|sc-69880|sc-69881|sc-69883|sc-10352|sc-137184|sc-137185 from Santa Cruz Biotechnology.|
|Validated Species||Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of mouse Growth hormone receptor(231-247aa DKEHEVRVRSRQRSFEK), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Growth hormone receptor(GH receptor)|
|Molecular Weight||72783 MW|
|Protein Function||Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to, and activates the JAK2/STAT5 pathway (By similarity). .|
|Tissue Specificity||Expressed in all tissues tested including, liver, heart, adipose tissue, mammary gland, testes, ovary, brain, kidney and muscle. Highest levels in liver.|
|Sequence Similarities||Belongs to the type I cytokine receptor family. Type 1 subfamily.|
|Subcellular Localization||Cell membrane; Single-pass type I membrane protein. On growth hormone binding, GHR is ubiquitinated, internalized, down-regulated and transported into a degradative or non-degradative pathway. .|
|Alternative Names||Growth hormone receptor;GH receptor;Somatotropin receptor;Growth hormone-binding protein;GH-binding protein;GHBP;Serum-binding protein;Ghr;|
|Research Areas|||neuroscience|neurotransmission|receptors / channels|gpcr|more gpcr| neuroscience|neurology process|neuroendocrinology|gh regulation||
Background for Growth hormone receptor(GH receptor)
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GHR/Growth Hormone R Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GHR/Growth Hormone R Antibody Images
Click the images to enlarge.
All lanes: Anti GHR (PA1726) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: Rat Intestine Tissue Lysate at 50ug
Lane 5: Mouse Spleen Tissue Lysate at 50ug
Lane 6: Mouse Testis Tissue Lysate at 50ug
Lane 7: Mouse Liver Tissue Lysate at 50ug
Lane 8: Mouse Kidney Tissue Lysate at 50ug
Lane 9: Mouse Intestine Tissue Lysate at 50ug
Predicted bind size: 72KD
Observed bind size: 72KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,