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GSTP1 antibody (PB9184) performs well in Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of THP-1 cells using anti-GST3/GST pi antibody (PB9184).
Overlay histogram showing THP-1 cells stained with PB9184 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GST3/GST pi Antibody (PB9184, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing THP-1 cells stained with PB9184 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GST3/GST pi Antibody (PB9184, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-GST3/GST pi/GSTP1 Antibody Picoband®
GSTP1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
GSTP1 antibody was positively detected by WB in:
human Hela whole cell, human 293T whole cell, huamn MDA-MB-231 whole cell, human HCC1937 whole cell, human Jurkat whole cell, mouse liver tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
GSTP1 antibody was positively detected by IHC in:
rat lung tissue, human lung cancer tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
GSTP1 antibody was positively detected by ICC/IF in:
SH-SY5Y cell
GSTP1 antibody was positively detected by FC in:
THP-1 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Recommend
Mouse
Tested
Rat
Tested
Recommend
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-GST3/GST pi/GSTP1 Antibody Picoband®
View all GSTP1 Antibodies
View all GSTP1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GST3 recombinant protein (Position: P2-Q210). Human GST3 shares 85% and 86% amino acid (aa) sequences identity with mouse and rat GST3, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
23.4 kDa
Background of GSTP1
Glutathione S-transferases pi (GSTP1), also known as GST3, is an enzyme that in humans is encoded by the GSTP1 gene. This gene is mapped to 11q13.2. GSTP1 has 7 exons and 6 introns contained within approximately 2.8 kilobases. GSTP1 belongs to Glutathione S-transferases (GSTs) which are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. GSTP1 presents in all tissues and cells, with the exception of red cells, in which only erythrocyte GST (GSTe) is observed. What's more, GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.
Cite This Product
Anti-GST3/GST pi/GSTP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9184)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Dog
87%
Moderate Homology
Cow
86%
Moderate Homology
Rat
86%
Moderate Homology
Rabbit
86%
Moderate Homology
Sheep
86%
Moderate Homology
Mouse
85%
Moderate Homology
Zebrafish
60%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for GSTP1 antibody
Immunohistochemistry (IHC) protocol for GSTP1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for GSTP1 antibody
Flow Cytometry (FC) protocol for GSTP1 antibody
Publications
Application:
Species:
Immunohistochemical investigation of GST-M, GST-P, and TP53 gene expression in obesity patients under laparoscopic sleeve gastrectomy
Published: 2025-09-16
Journal: TISSUE & CELL
Application: IHC
Impact Factor: 2.50
Species: Human
PMID: 40967125
The Herbal Constituents in An-Gong-Niu-Huang Wan (AGNH) Protect against Cinnabar- and Realgar-Induced Hepatorenal Toxicity and Accumulations of Mercury and Arsenic in Mice
Published: 2021-04-02
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 2.63
Species: Mouse
PMID: 33868437
The recovery trajectory of adolescent social defeat stress-induced behavioral, 1H-MRS metabolites and myelin changes in Balb/c mice
Published: 2016-06-10
Journal: Scientific Reports
Application: IHC
Impact Factor: 5.23
Species: Mouse
PMID: 27283029
Overdose Intake of Curcumin Initiates the Unbalanced State of Bodies
Published: 2016-03-25
Journal: JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Application: WB
Impact Factor: 2.86
Species: Rat
PMID: 26978516
Toxicological risks of Rhizoma paridis saponins in rats involved NF-κB and Nrf2 signaling
Published: 2016-03-15
Journal: RSC Advances
Application: WB
Impact Factor: 3.29
Species: Rat
Quetiapine mitigates the neuroinflammation and oligodendrocyte loss in the brain of C57BL/6 mouse following cuprizone exposure for one week
Published: 2015-08-28
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IHC
Impact Factor: 2.53
Species: Mouse
PMID: 26321148
Ligustrazine derivate DLJ14 reduces multidrug resistance of K562/A02 cells by modulating GSTπ activity
Published: 2011-03-12
Journal: TOXICOLOGY IN VITRO
Application: WB
Impact Factor: 2.55
Species: Human
PMID: 21402141
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Product Promise
We promise Anti-GST3/GST pi/GSTP1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9184
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 10, 2026