Product Info Summary
SKU: | CI1078 |
---|---|
Size: | 50μg/54μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ChIP, ChIP-seq, Dot blot, ELISA, IF, WB |
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Product info
Product Name
Anti-H2AK5ac HIST1H2AB Antibody
SKU/Catalog Number
CI1078
Size
50μg/54μl
Form
Liquid
Description
Boster Bio Anti-H2AK5ac HIST1H2AB Antibody (Catalog# CI1078). Tested in ChIP, ChIP-seq, ELISA, Dot blot, WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-H2AK5ac HIST1H2AB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1078)
Host
Rabbit
Contents
Affinity purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against the region of histone H2A containing the acetylated lysine 5 (H2AK5ac), using a KLH-conjugated synthetic peptide.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1078 is reactive to HIST3H2A in Human
Applications
CI1078 is guaranteed for ChIP, ChIP-seq, Dot blot, ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
14.121kDa
Background of HIST3H2A
Histones are the main constituents of the protein part of chromosomes of eukaryotic cells. They are rich in the amino acids arginine and lysine and have been greatly conserved during evolution. Histones pack the DNA into tight masses of chromatin. Two core histones of each class H2A, H2A, H3 and H4 assemble and are wrapped by 146 base pairs of DNA to form one octameric nucleosome. Histone tails undergo numerous post-translational modifications, which either directly or indirectly alter chromatin structure to facilitate transcriptional activation or repression or other nuclear processes. In addition to the genetic code, combinations of the different histone modifications reveal the so-called “histone code”. Histone methylation and demethylation is dynamically regulated by respectively histone methyl transferases and histone demethylases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
ChIP assays were performed using human HeLa cells, Anti-H2AK5ac polyclonal antibody (Catalog # CI1078) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 0.5, 1, 2 and, 5 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for a region approximately 1 kb upstream of the GAPDH and ACTB promoters, used as positive controls, and for the coding region of the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls.
Click image to see more details
To determine the titer of the antibody, an ELISA was performed using a serial dilution of Anti-H2AK5ac polyclonal antibody (Catalog # CI1078) in antigen coated wells. The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:25,000.
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ChIP was performed on sheared chromatin from 1.5 million HeLaS3 cells using 0.5 μg of Anti-H2AK5ac polyclonal antibody (Catalog # CI1078). The IP DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the enrichment along the complete sequence and a 1 Mb region of the X-chromosome (fig 2A and B) and in genomic regions of chromosome 7, surrounding the ACTB gene, and of chromosome 12, surrounding the GAPDH gene (fig 2C and D). The position of the amplicon used for ChIP-qPCR is indicated by an arrow.
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Western blot analysis of H2AK5ac expression in HeLa whole cell lysates (25 μg, lane 1), histone extracts from HeLa cells (15 μg, lane 2), recombinant histone H2A (1 μg, lane 3), recombinant histone H2B (1 μg, lane 4), recombinant histone H3 (1 μg, lane 5) and recombinant histone H4 (1 μg, lane 6). H2AK5ac was detected using Anti-H2AK5ac polyclonal antibody (Catalog # CI1078) at 1/1000 dilution.
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A Dot Blot analysis was performed to test the cross reactivity of Anti-H2AK5ac polyclonal antibody (Catalog # CI1078) with peptides containing other histone modifications and the unmodified H2AK5. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. The antibody was used at a dilution of 1:5,000. This figure shows a high specificity of the antibody for the modification of interest.
Protein Target Info & Infographic
Gene/Protein Information For HIST3H2A (Source: Uniprot.org, NCBI)
Gene Name
HIST3H2A
Full Name
Histone H2A type 3
Weight
14.121kDa
Superfamily
histone H2A family
Alternative Names
Histone H2A type 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIST3H2A, check out the HIST3H2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIST3H2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-H2AK5ac HIST1H2AB Antibody (CI1078)
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No publications found for CI1078
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