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Product Info Summary
| SKU: | A01073-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-Hck Monoclonal Antibody
SKU/Catalog Number
A01073-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-Hck Monoclonal Antibody catalog # A01073-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hck Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01073-1)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide, 0.5% stabilizing protein, 50% glycerol
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
3D12E1
Isotype
IgG1
Immunogen
Purified recombinant fragment of Hck expressed in E. Coli.
Reactive Species
A01073-1 is reactive to HCK in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
59.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01073-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
ELISA 1:10000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human colon cancer (left) and ancreas cancer (right), showing cytoplasmic localization with DAB staining using Hck Monoclonal Antibody.
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Western Blot analysis using Hck Monoclonal Antibody against truncated HCK recombinant protein (1) and full-length HCK-GFP transfected CHO-K1 cell lysate (2).
Specific Publications For Anti-Hck Monoclonal Antibody (A01073-1)
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Customer Q&As
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1 Customer Q&As for Anti-Hck Monoclonal Antibody
Question
We are currently using anti-Hck Monoclonal antibody A01073-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
B. Taylor
Verified customer
Asked: 2013-10-15
Answer
The anti-Hck Monoclonal antibody (A01073-1) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-15
