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Product Info Summary
| SKU: | M00595-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5]
SKU/Catalog Number
M00595-2
Size
100 µl
Description
Boster Bio HDAC6 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5). Catalog# M00595-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00595-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI4C5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human HDAC6 (NP_006035) produced in HEK293T cell.
Reactive Species
M00595-2 is reactive to HDAC6 in Dog, Human, Monkey, Rat
Calculated molecular weight
131.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00595-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000~2000
IF 1:100
Flow Cytometry 1:100
IHC 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC6.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HDAC6 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HDAC6 mouse monoclonal antibody (M00595-2) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (M00595-2) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (M00595-2) at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HDAC6 mouse monoclonal antibody (M00595-2) at 1:150 dilution.
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HEK293T cells transfected with either HDAC6 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HDAC6 antibody (M00595-2)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-HDAC6 mouse monoclonal antibody (M00595-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HDAC6.
Specific Publications For Anti-HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5] (M00595-2)
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