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Product Info Summary
| SKU: | M05215 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-HDAC10 Rabbit Monoclonal Antibody
SKU/Catalog Number
M05215
BM4720 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-HDAC10 Rabbit Monoclonal Antibody catalog # M05215. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HDAC10 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05215)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
HEO-8
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human HDAC10
Reactive Species
M05215 is reactive to HDAC10 in Human, Mouse
Observed Molecular Weight
75 kDa
Calculated molecular weight
71.4 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05215 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Positive Control
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HDAC10 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate.
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Immunofluorescent analysis of Hela cells, using HDAC10 Antibody.
Specific Publications For Anti-HDAC10 Rabbit Monoclonal Antibody (M05215)
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Customer Q&As
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5 Customer Q&As for Anti-HDAC10 Rabbit Monoclonal Antibody
Question
Is this M05215 anti-HDAC10 Rabbit Monoclonal antibody reactive to the isotypes of HDAC10?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
The immunogen of M05215 anti-HDAC10 Rabbit Monoclonal antibody is A synthesized peptide derived from human HDAC10 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-06
Question
I would like to test anti-HDAC10 Rabbit Monoclonal antibody M05215 on human amygdala for research purposes, then I may be interested in using anti-HDAC10 Rabbit Monoclonal antibody M05215 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The products we sell, including anti-HDAC10 Rabbit Monoclonal antibody M05215, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-04
Question
Is a blocking peptide available for product anti-HDAC10 Rabbit Monoclonal antibody (M05215)?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
We do provide the blocking peptide for product anti-HDAC10 Rabbit Monoclonal antibody (M05215). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-06
Question
Does anti-HDAC10 Rabbit Monoclonal antibody M05215 work on dog WB with leukemia?
Verified Customer
Verified customer
Asked: 2017-08-28
Answer
Our lab technicians have not tested anti-HDAC10 Rabbit Monoclonal antibody M05215 on dog. You can run a BLAST between dog and the immunogen sequence of anti-HDAC10 Rabbit Monoclonal antibody M05215 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog leukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-28
Question
I see that the anti-HDAC10 Rabbit Monoclonal antibody M05215 works with IP, what is the protocol used to produce the result images on the product page?
S. Mitchell
Verified customer
Asked: 2017-07-05
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-05
