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Product Info Summary
| SKU: | M00013-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242
SKU/Catalog Number
M00013-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242 (Catalog # M00013-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00013-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% stabilizing protein and 0.09% sodium azide
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
RM242
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to Hypoxia-inducible factor 1-alpha
Cross-reactivity
This antibody reacts to human HIF-1-alpha (Hypoxia-inducible factor 1-alpha).
Reactive Species
M00013-1 is reactive to HIF1A in Human
Calculated molecular weight
92.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00013-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Western Blotting result
Western Blot of Jurkat cell lysate, nontreated or treated with Cobalt(II) chloride (CoCl2), using Anti-HIF-1-alpha RM242 at a 1:1000 dilution, showed that HIF-1-alpha (~120 kDa) expression was induced by CoCl2 in Jurkat cells.
Click image to see more details
IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-HIF-1-alpha RM242 at a 1:1000 dilution.
Specific Publications For Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242 (M00013-1)
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Customer Q&As
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16 Customer Q&As for Anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal Antibody, Clone#RM242
Question
We ordered your anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
I viewed the website and datasheets of our anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-27
Question
I see that the anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-12-05
Question
Is there a BSA free version of anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 available?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-20
Question
We are currently using anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 (M00013-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
I was wanting to use your anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 for WB for human glial tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human glial tumor identification?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
You can see on the product datasheet, M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human glial tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-08
Question
Would anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 work for WB with glial tumor?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
According to the expression profile of glial tumor, HIF1A is highly expressed in glial tumor. So, it is likely that anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 will work for WB with glial tumor.
Boster Scientific Support
Answered: 2019-07-08
Question
Is this M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 reactive to the isotypes of HIF1A?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
The immunogen of M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 is A peptide corresponding to Hypoxia-inducible factor 1-alpha. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-20
Question
We are interested in using your anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 for positive regulation of vascular endothelial growth factor receptor signaling pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-10
Answer
We appreciate your inquiry. This M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 is validated on jurkat cell lysate, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-10
Question
Our lab were content with the WB result of your anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-12-18
Answer
From literature, hepatoma does express HIF1A. Generally HIF1A expresses in cytoplasm. Regarding which tissues have HIF1A expression, here are a few articles citing expression in various tissues:
Choriocarcinoma, and Placenta, Pubmed ID: 15489334
Hepatoma, Pubmed ID: 9079689
Liver, Pubmed ID: 12508121
Boster Scientific Support
Answered: 2018-12-18
Question
I have a question about product M00013-1, anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-29
Question
We have been able to see staining in human glial tumor. Do you have any suggestions? Is anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 supposed to stain glial tumor positively?
B. Jones
Verified customer
Asked: 2018-07-17
Answer
Based on literature glial tumor does express HIF1A. Based on Uniprot.org, HIF1A is expressed in visceral pleura, hepatoma, glial tumor, liver, choriocarcinoma placenta, among other tissues. Regarding which tissues have HIF1A expression, here are a few articles citing expression in various tissues:
Choriocarcinoma, and Placenta, Pubmed ID: 15489334
Hepatoma, Pubmed ID: 9079689
Liver, Pubmed ID: 12508121
Boster Scientific Support
Answered: 2018-07-17
Question
Will M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-23
Answer
You can see on the product datasheet, M00013-1 anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-23
Question
I have attached the WB image, lot number and protocol we used for glial tumor using anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-05-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-11
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for glial tumor using anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1. Let me know if you need anything else.
E. Li
Verified customer
Asked: 2017-05-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-01
Question
Is a blocking peptide available for product anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 (M00013-1)?
J. Wu
Verified customer
Asked: 2014-12-22
Answer
We do provide the blocking peptide for product anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 (M00013-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2014-12-22
Question
My lab would like to test anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 on human glial tumor for research purposes, then I may be interested in using anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Miller
Verified customer
Asked: 2014-09-22
Answer
The products we sell, including anti-HIF-1 alpha (HIF1A) Rabbit Monoclonal antibody, Clone#RM242 M00013-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-09-22
