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Product Info Summary
| SKU: | M00013-2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Monkey, Mouse |
| Host: | Mouse |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-HIF-alpha HIF1A Monoclonal Antibody
SKU/Catalog Number
M00013-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-HIF-alpha HIF1A Monoclonal Antibody catalog # M00013-2. Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-alpha HIF1A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00013-2)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
1A3
Isotype
IgG
Immunogen
Purified recombinant fragment of human HIF-1α expressed in E. Coli.
Reactive Species
M00013-2 is reactive to HIF1A in Human, Monkey, Mouse
Calculated molecular weight
92.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00013-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
ELISA 1:10000
Validation Images & Assay Conditions
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Western blotting validation for Anti-HIF-alpha HIF1A Monoclonal Antibody M00013-2
Western Blot (WB) analysis using HIF-1alpha Monoclonal Antibody against Cos7 (1)
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Immunohistochemistry validation of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).
Immunohistochemistry (IHC) analysis of paraffin-embedded liver cancer tissues (left) and Lung Cancer Tissues (right) with DAB staining using HIF-1alpha Monoclonal Antibody.For more protocol information of IHC
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Immunofluorescent staining data of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).
Immunofluorescence (IF) analysis of HeLa cells using HIF-1alpha Monoclonal Antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA validation of HIF1A using Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2).
ELISA analysis of HIF-1alpha antibody.Specific Publications For Anti-HIF-alpha HIF1A Monoclonal Antibody (M00013-2)
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