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Product Info Summary
| SKU: | M00013-3 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-HIF-1 alpha Rabbit Monoclonal Antibody
View all HIF-1 alpha Antibodies
SKU/Catalog Number
M00013-3
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-HIF-1 alpha Rabbit Monoclonal Antibody catalog # M00013-3. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HIF-1 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00013-3)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
17H18
Isotype
IgG
Immunogen
A synthesized peptide derived from human HIF-1 alpha
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00013-3 is reactive to HIF1A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
127 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00013-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:30
FC 1:30
Positive Control
IHC: human kindey tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of HIF-1 alpha expression in Ramos cell lysate.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human kindey, using HIF-1 alpha Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunofluorescent analysis of Hela cells, using HIF-1 alpha Antibody.
Protein Target Info & Infographic
Gene/Protein Information For HIF1A (Source: Uniprot.org, NCBI)
Gene Name
HIF1A
Full Name
Hypoxia-inducible factor 1-alpha
Weight
88068 MW
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; HIF1A HIF-1-alpha, HIF-1A, HIF-1alpha, HIF1, HIF1-ALPHA, MOP1, PASD8, bHLHe78 hypoxia inducible factor 1 subunit alpha hypoxia-inducible factor 1-alpha|ARNT interacting protein|PAS domain-containing protein 8|basic-helix-loop-helix-PAS protein MOP1|class E basic helix-loop-helix protein 78|hypoxia inducible factor 1 alpha subunit|hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)|hypoxia-inducible factor1alpha|member of PAS protein 1|member of PAS superfamily 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HIF1A, check out the HIF1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HIF1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF-1 alpha Rabbit Monoclonal Antibody (M00013-3)
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