Anti-HIF-1 alpha Rabbit Monoclonal Antibody

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of HIF-1 alpha expression in Ramos cell lysate.

Activation of HIF-1α/BNIP3 signaling pathway in artery of KD murine model. A Representative immumohistochemical images for HIF-1α expression in aorta ( n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. B Representative immumohistochemical images for HIF-1α expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm. C Representative immumohistochemical images for BNIP3 expression in aorta ( n = 4 per group). Enlarged images of area of interesting (AOI) were indicated with red block diagrams. Scale bar: 50 μm. D Representative immumohistochemical images for BNIP3 expression in coronary bifurcation arteritis ( n = 4 per group). Scale bar: 50 μm.
Index in PubMed under a CC BY license. PMID: 37980402

Short-term DMOG treatment reduces MSC senescence by activating HIF-1α and decreasing apoptosis. ( A , B ) Representative images of SA-β-gal staining showing the proportion of senescent cells in H₂O₂-treated MSCs before and after DMOG treatment. Scale bar = 500 μm. ( C ) Western blot analysis of p53 and p21 protein expression during oxidative stress-induced senescence. ( D , E ) SA-β-gal staining in replicative senescence (P15) MSCs, demonstrating the effect of DMOG in reducing senescence markers. Scale bar = 500 μm. ( F ) Western blot analysis of p53 and p21 protein levels in P5 (young) and P15 (senescent) MSCs. ( G ) Expression levels of senescence-associated genes (IL6, CXCL1, and MMP3) in both senescence models as assessed by qRT-PCR. ( H ) Western blotting and qPCR analysis showing increased HIF-1α protein and mRNA levels in both senescence models after DMOG treatment. ( I ) Calcein/PI live-dead staining revealed an increase in the proportion of live cells following DMOG treatment in both senescence models. ( J , K ) Flow cytometric analysis of apoptosis. Error bars represent the mean ± SD of three independent experiments. Statistical significance was set as p < 0.05. Full-length blots are presented in Supplementary Materials - WB Raw Data Full size image
Index in PubMed under a CC BY license. PMID: 40457488

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human kindey, using HIF-1 alpha Antibody.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunofluorescent analysis of Hela cells, using HIF-1 alpha Antibody.

Western blot analysis of HIF-1 alpha using anti-HIF-1 alpha antibody (M00013-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1-5: model group-human uterine tissue lysates,
Lane 6-10: young group-human uterine tissue- lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HIF-1 alpha antigen affinity purified monoclonal antibody (A04887-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at for 1 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for HIF-1 alpha at approximately 120 kDa. The expected band size for HIF-1 alpha is at 120 kDa.