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- Table of Contents
HIF1A antibody (M00013-3) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis of Hela cells, using HIF-1 alpha Antibody.
Designing a Western blot for HIF1A? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the HIF1A Western Blot GuideBundle Save
Anti-HIF-1 alpha Rabbit Monoclonal Antibody
HIF1A antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
HIF1A antibody was positively detected by IHC in:
human kindey tissue
Use EDTA pH 8.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
HIF1A antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:30
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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Boster Guarantee:What is this?
WB
IHC
IF
IP
FC
Human
Recommend
Tested
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-HIF-1 alpha Rabbit Monoclonal Antibody
View all HIF1A Antibodies
View all HIF1A Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
17H18
Isotype
IgG
Immunogen
A synthesized peptide derived from human HIF-1 alpha
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
120 kDa
Calculated Molecular Weight
92.7 kDa
Cite This Product
Anti-HIF-1 alpha Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00013-3)
Protocols
Immunohistochemistry (IHC) protocol for HIF1A antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for HIF1A antibody
Publications
Application:
Species:
Hypoxia-Induced Extracellular Vesicles Derived from Human Umbilical Cord Mesenchymal Stem Cells Regulate Macrophage Polarization and Enhance Angiogenesis to Promote Diabetic Wound Healing
Published: 2025-10-24
Journal: Biomolecules
Application: IF,WB
Impact Factor: 4.80
Species: Human
Short-Term DMOG treatment rejuvenates senescent mesenchymal stem cells by enhancing mitochondrial function and mitophagy through the HIF-1α/BNIP3 pathway
Published: 2025-06-02
Journal: Stem Cell Research & Therapy
Application: WB
Impact Factor: 7.30
Species: Human
PMID: 40457488
Optimizing interventional therapy: A homogeneous lipiodol formulation of Tirapazamine and Sorafenib responsive to post-embolization microenvironment
Published: 2025-01-30
Journal: JOURNAL OF CONTROLLED RELEASE
Application: WB,IHC
Impact Factor: 11.50
Species: Rat,Human
PMID: 39880038
Hypoxia Reversion and STING Pathway Activation through Large Mesoporous Nanozyme for Near-Infrared-II Light Amplified Tumor Polymetallic-Immunotherapy
Published: 2024-08-09
Journal: ACS Nano
Application: IHC
Impact Factor: 15.80
Species: Mouse
PMID: 39118372
NIR-Mediated Pyroelectric Catalysis for Sustained ROS/RNS Generation and Advanced Cancer Therapy In Vivo via Injectable Hydrogel
Published: 2024-07-23
Journal: ACS Applied Materials & Interfaces
Application: WB
Impact Factor: 8.30
Species: Human
PMID: 39039973
Prognostic implications of TOR1B expression across cancer types: a focus on basal-like breast cancer and cellular adaptations to hypoxia
Published: 2024-06-06
Journal: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
Application: WB
Impact Factor: 2.70
Species: Human
PMID: 38842687
Liensinine sensitizes colorectal cancer cells to oxaliplatin by targeting HIF-1a to inhibit autophagy
Published: 2024-04-22
Journal: PHYTOMEDICINE
Application: WB,IHC
Impact Factor: 6.70
Species: Mouse
PMID: 38703660
Inhibition of PDGFRβ alleviates endothelial cell apoptotic injury caused by DRP-1 overexpression and mitochondria fusion failure after mitophagy
Published: 2023-11-18
Journal: Cell Death & Disease
Application: IHC
Impact Factor: 9.00
Species: Mouse
PMID: 37980402
Explore the mechanism and substance basis of Mahuang FuziXixin Decoction for the treatment of lung cancer based on network pharmacology and molecular docking
Published: 2022-11-10
Journal: COMPUTERS IN BIOLOGY AND MEDICINE
Application: WB
Impact Factor: 6.70
Species: Human
PMID: 36399857
Salidroside attenuates CoCl2-simulated hypoxia injury in PC12 cells partly by mitochondrial protection
Published: 2021-11-05
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB,IF
Impact Factor: 4.43
Species: Rat
PMID: 34748770
A novel role of HIF-1α/PROX-1/LYVE-1 axis on tissue regeneration after renal ischaemia/reperfusion in mice
Published: 2018-04-10
Journal: ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY
Application: IHC,IF
Impact Factor: 1.81
Species: Mouse
PMID: 29633855
IL-17-triggered downregulation of miR-497 results in high HIF-1α expression and consequent IL-1β and IL-6 production by astrocytes in EAE mice
Published: 2017-05-01
Journal: Cellular & Molecular Immunology
Application: WB,ChIP
Impact Factor: 5.90
Species: Mouse
PMID: 28458392
Review
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1 Review For Anti-HIF-1 alpha Rabbit Monoclonal Antibody
The HIF1A antibody was used to detect the expression of the target protein in human uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse.
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE. |
| Primary Antibody | Anti-HIF-1 alpha Rabbit Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system:Tanon |
| Results Summary | The HIF1A antibody was used to detect the expression of the target protein in human uterine tissue. The Western blot results showed clear bands, and the antibody maintained good performance after reuse. It offers excellent cost-effectiveness, with a clear advantage over other antibodies with similar specifications. Highly recommended for use! |
Anfeng Ning, Peking University Third Hospital
Verified customer
Submitted 2025-11-06
Product Promise
We promise Anti-HIF-1 alpha Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00013-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 19, 2026