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Product Info Summary
| SKU: | M02263-2 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1]
View all ARNT/HIF-1 beta Antibodies
SKU/Catalog Number
M02263-2
Size
100 µl
Description
Boster Bio ARNT (HIF1 beta) mouse monoclonal antibody, clone OTI1A1 (formerly 1A1). Catalog# M02263-2. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02263-2)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1A1
Isotype
IgG1
Immunogen
Full length human recombinant protein of human ARNT (NP_001659) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02263-2 is reactive to ARNT in Human, Mouse, Rat
Applications
M02263-2 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
87 kDa
Calculated molecular weight
86.636kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNT.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-ARNT monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-ARNT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either ARNT (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ARNT antibody (M02263-2)
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Flow cytometric Analysis of Jurkat cells
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Anti-ARNT mouse monoclonal antibody (M02263-2) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNT.
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Figure from citation: Immunofluorescence of ARNT protein level by using anti-ARNT antibody in mouse neuronal cells.
Protein Target Info & Infographic
Gene/Protein Information For ARNT (Source: Uniprot.org, NCBI)
Gene Name
ARNT
Full Name
Aryl hydrocarbon receptor nuclear translocator
Weight
86.636kDa
Alternative Names
ARNT protein; ARNT; aryl hydrocarbon receptor nuclear translocator; BHLHE2; HIF1 beta; HIF-1 beta; HIF1B; HIF1BETA; HIF-1-beta; HIF1-beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; nuclear translocator; TANGO ARNT HIF-1-beta, HIF-1beta, HIF1-beta, HIF1B, HIF1BETA, TANGO, bHLHe2 aryl hydrocarbon receptor nuclear translocator aryl hydrocarbon receptor nuclear translocator|class E basic helix-loop-helix protein 2|dioxin receptor, nuclear translocator|hypoxia-inducible factor 1, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARNT, check out the ARNT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARNT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HIF1 beta (ARNT) Mouse Monoclonal Antibody [Clone ID: OTI1A1] (M02263-2)
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