Anti-Beclin 1/BECN1 Antibody Picoband™

Beclin 1 antibody

Boster Bio Anti-Beclin 1/BECN1 Antibody Picoband™ catalog # PB9076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 10 publication(s).

Product Info Summary

SKU: PB9076
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-Beclin 1/BECN1 Antibody Picoband™

View all Beclin 1 Antibodies

SKU/Catalog Number

PB9076

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Beclin 1/BECN1 Antibody Picoband™ catalog # PB9076. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Beclin 1/BECN1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9076)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9076 is reactive to BECN1 in Human, Mouse, Rat

Applications

PB9076 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

51.896kDa

Background of Beclin 1

Beclin-1, also known as also known as ATG6 or VPS30 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. This gene participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of the affected which causes diminished autophagywhich in turn results in increased neuronal cell death. It has been found that beclin-1 can promote autophagy in autophagy-defective yeast with a targeted disruption of apg6/vps30, and in human MCF7 breast carcinoma cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For BECN1 (Source: Uniprot.org, NCBI)

Gene Name

BECN1

Full Name

Beclin-1

Weight

51.896kDa

Superfamily

beclin family

Alternative Names

ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30 BECN1 ATG6, VPS30, beclin1 beclin 1 beclin-1|ATG6 autophagy related 6 homolog|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)|beclin 1, autophagy related|coiled-coil myosin-like BCL2-interacting protein|testis secretory sperm-binding protein Li 215e

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on BECN1, check out the BECN1 Infographic

BECN1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for BECN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9076 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The Protein-Independent Role of Phosphate in the Progression of Chronic Kidney Disease

Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo

MicroRNA 30a Mediated Autophagy and Imatinib Response in Egyptian Chronic Myeloid Leukemia Patients

Endogenous dipeptide-carnosine supplementation ameliorates hypobaric hypoxia-induced skeletal muscle loss via attenuating endoplasmic reticulum stress response and maintaining proteostasis

Gang Zhong,Huiping Long,Shiting Ma,Yao Shunhan,Jia Li,Jun Yao,miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis,Life Sciences,Volume 226,2019,Pages 164-172,ISSN 0024-3205,https://doi.org/10.1016/j. lfs.2019.03.071.
Species: Human
PB9076 usage in article: APP:WB, SAMPLE:CHONDROCYTES, DILUTION:NA

Zhiwei Liao,Suyun Li,Rong Liu,Xiaobo Feng,Yunsong Shi,Kun Wang,Shuai Li,Yukun Zhang,Xinghuo Wu,Cao Yang,"Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo", Oxidative Medicine and Cellular Longevity,vol.2021,Article ID 5584447,22 pages,2021.https://doi.org/10.1155/2021/5584447
Species: Human,Rat
PB9076 usage in article: APP:WB, SAMPLE:NP CELL, DILUTION:1:1000

Ni K,Hua Y.Hydrogen sulfide exacerbated periodontal inflammation and induced autophagy in experimental periodontitis.Int Immunopharmacol.2021 Jan 30;93:107399.doi:10.1016/j.intimp.2021.107399.Epub ahead of print.PMID:33529908.
Species: Human,Rat
PB9076 usage in article: APP:IF, SAMPLE:ALVEOLAR BONE SPECIMEN, DILUTION:1:500

Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
PB9076 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:400

Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy

Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy

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15 Customer Q&As for Anti-Beclin 1/BECN1 Antibody Picoband™

Question

We have observed staining in mouse cervix. Any tips? Is anti-Beclin 1/BECN1 antibody supposed to stain cervix positively?

Verified Customer

Verified customer

Asked: 2020-01-16

Answer

From what I have seen in literature cervix does express BECN1. From what I have seen in Uniprot.org, BECN1 is expressed in frontal cortex, brain, liver, cervix, mammary gland, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091

Boster Scientific Support

Answered: 2020-01-16

Question

We need to test anti-Beclin 1/BECN1 antibody PB9076 on rat liver for research purposes, then I may be interested in using anti-Beclin 1/BECN1 antibody PB9076 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-15

Answer

The products we sell, including anti-Beclin 1/BECN1 antibody PB9076, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-15

Question

My question regards using your anti-Beclin 1/BECN1 antibody for apoptotic process studies. Has this antibody been tested with western blotting on rat spleen tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

We appreciate your inquiry. This PB9076 anti-Beclin 1/BECN1 antibody is tested on mouse spleen tissue, intestine tissue, rat spleen tissue, colo320 whole cell lysate, hepg2 whole cell lysate, panc whole cell lysate, a431 whole cell lysate, smmc whole cell lysate, jurkat whole cell lysate, sw620 whole cell lysate, u87 whole cell lysate. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-12-27

Question

We purchased anti-Beclin 1/BECN1 antibody for Flow Cytometry on mammary gland in a previous project. I am using mouse, and We intend to use the antibody for WB next. We are interested in examining mammary gland as well as cervix carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

I looked at the website and datasheets of our anti-Beclin 1/BECN1 antibody and I see that PB9076 has been validated on mouse in both Flow Cytometry and WB. Thus PB9076 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-03

Question

Is a blocking peptide available for product anti-Beclin 1/BECN1 antibody (PB9076)?

Verified Customer

Verified customer

Asked: 2019-10-30

Answer

We do provide the blocking peptide for product anti-Beclin 1/BECN1 antibody (PB9076). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-10-30

Question

Does anti-Beclin 1/BECN1 antibody PB9076 work for WB with liver?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

According to the expression profile of liver, BECN1 is highly expressed in liver. So, it is likely that anti-Beclin 1/BECN1 antibody PB9076 will work for WB with liver.

Boster Scientific Support

Answered: 2019-10-08

Question

I see that the anti-Beclin 1/BECN1 antibody PB9076 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-05-17

Question

My team were satisfied with the WB result of your anti-Beclin 1/BECN1 antibody. However we have seen positive staining in cervix cytoplasm using this antibody. Is that expected? Could you tell me where is BECN1 supposed to be expressed?

M. Anderson

Verified customer

Asked: 2019-01-11

Answer

Based on literature, cervix does express BECN1. Generally BECN1 expresses in cytoplasm. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091

Boster Scientific Support

Answered: 2019-01-11

Question

Can you help my question with product PB9076, anti-Beclin 1/BECN1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-12-20

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9076 anti-Beclin 1/BECN1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-12-20

Question

Does PB9076 anti-Beclin 1/BECN1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2018-03-23

Answer

As indicated on the product datasheet, PB9076 anti-Beclin 1/BECN1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-03-23

Question

Please see the WB image, lot number and protocol we used for liver using anti-Beclin 1/BECN1 antibody PB9076. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-05

Question

Do you have a BSA free version of anti-Beclin 1/BECN1 antibody PB9076 available?

Verified Customer

Verified customer

Asked: 2017-06-27

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Beclin 1/BECN1 antibody PB9076 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-06-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Beclin 1/BECN1 antibody PB9076. Let me know if you need anything else.

A. Lewis

Verified customer

Asked: 2015-12-31

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-31

Question

We are currently using anti-Beclin 1/BECN1 antibody PB9076 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

R. Gonzalez

Verified customer

Asked: 2014-11-17

Answer

The anti-Beclin 1/BECN1 antibody (PB9076) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-11-17

Question

Is this PB9076 anti-Beclin 1/BECN1 antibody reactive to the isotypes of BECN1?

R. Parker

Verified customer

Asked: 2013-07-05

Answer

The immunogen of PB9076 anti-Beclin 1/BECN1 antibody is E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-07-05

Order DetailsPrice
PB9076

100μg

$370
PB9076-10ug

10μg sample (liquid)

$99
PB9076-Biotin

100 μg Biotin conjugated

$570
PB9076-Cy3

100 μg Cy3 conjugated

$570
PB9076-Dylight488

100 μg Dylight488 conjugated

$570
PB9076-Dylight550

100 μg Dylight550 conjugated

$570
PB9076-Dylight594

100 μg Dylight594 conjugated

$570
PB9076-FITC

100 μg FITC conjugated

$570
PB9076-HRP

100 μg HRP conjugated

$570
PB9076-APC

100 μg APC conjugated

$670
PB9076-PE

100 μg PE conjugated

$670
PB9076-carrier-free

Carrier Free

$370

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PB9076
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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