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BECN1 antibody (PB9076) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 44 Publications

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pb9076 beclin 1 primary antibodies fcm testing 3

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Flow Cytometry analysis of Raji cells using anti-Beclin 1 antibody (PB9076).
Overlay histogram showing Raji cells stained with PB9076 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beclin 1 Antibody (PB9076, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Designing a Western blot for BECN1? The guide covers the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.

Open the BECN1 Western Blot Guide

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Anti-Beclin 1/BECN1 Antibody Picoband®

BECN1 antibody

44 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IP, IF, ICC, WB

Tested Applications

BECN1 antibody was positively detected by WB in:

human Raji whole cell lysates, human 293T whole cell lysates, human MCF-7 whole cell lysates, human PC-3 whole cell lysates, rat C6 whole cell lysates, rat PC-12 whole cell lysates, mouse NIH/3T3 whole cell lysates, mouse RAW264.7 whole cell lysates

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

BECN1 antibody was positively detected by ICC/IF in:

Hela cell

BECN1 antibody was positively detected by FC in:

Raji cell

BECN1 antibody was positively detected by IP in:

MCF-7 whole cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

Human

Tested

Rat

Tested

Mouse

Tested

Monkey

Predicted

Bovine

Predicted

Sheep

Predicted

Dog

Predicted

Rabbit

Predicted

Product Information

Product Name

Anti-Beclin 1/BECN1 Antibody Picoband®
View all BECN1 Antibodies

Form

Lyophilized

Uniprot

Q14457

Gene ID

8678

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human Beclin 1 recombinant protein (Position: M1-S354). Human Beclin 1 shares 97% amino acid (aa) sequence identity with both mouse and rat Beclin 1.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

60 kDa

Calculated Molecular Weight

51.9 kDa

Background of BECN1

Beclin-1, also known as also known as ATG6 or VPS30 is a protein that in humans is encoded by the BECN1 gene. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagosome in autophagy. This gene participates in the regulation of autophagy and has an important role in development, tumorigenesis, and neurodegeneration. Schizophrenia is associated with low levels of Beclin-1 in the hippocampus of the affected which causes diminished autophagywhich in turn results in increased neuronal cell death. It has been found that beclin-1 can promote autophagy in autophagy-defective yeast with a targeted disruption of apg6/vps30, and in human MCF7 breast carcinoma cells.

Cite This Product

Anti-Beclin 1/BECN1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9076)

Protocols

Western Blot protocol for BECN1 antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BECN1 antibody

Review Protocol

Flow Cytometry (FC) protocol for BECN1 antibody

Review Protocol

Immunoprecipitation (IP) protocol for BECN1 antibody

Review Protocol

Publications

Gypenoside XVII protects against cisplatin-induced acute kidney injury by activating PINK1/Parkin-mediated mitophagy

Published: 2026-03-11

Journal: Asian Pacific Journal of Tropical Biomedicine

Application: WB

Impact Factor: 1.70

Species: Rat

PMID: 36250441

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Anti-Beclin 1/BECN1 Antibody Picoband®

BECN1 antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Gypenoside XVII protects against cisplatin-induced acute kidney injury by activating PINK1/Parkin-mediated mitophagy

Exercise-induced irisin ameliorates cognitive impairment following chronic cerebral hypoperfusion by suppressing neuroinflammation and hippocampal neuronal apoptosis

Hypoxia-induced autophagy attenuates ferredoxin 1-mediated cuproptosis in colorectal cancer cells

Deletion of p75NTR rescues behavioral and cognitive dysfunction in SPS-induced PTSD mice through hippocampal PI3K/Akt/mTOR pathway

Aconiti Lateralis Radix Praeparata ameliorates heart failure via PI3K/AKT/Bnip3 pathway

The Therapeutic Mechanisms of Shenyan Oral Liquid I Against Chronic Kidney Disease Based on Network Pharmacology and Experimental Validation

p75NTR mediated chronic restraint stress-induced depression-like behaviors in mice via hippocampal mTOR pathway

Lhx6 deficiency causes human embryonic palatal mesenchymal cell mitophagy dysfunction in cleft palate

The Role of Autophagy on Osteogenesis of Dental Follicle Cells Under Inflammatory Microenvironment

Irisin alleviates the pyroptosis of β cells in T2DM by inhibiting NLRP3 inflammasome through regulating miR-19b-3p/SOCS3/STAT3 axis mediated autophagy

The role of osteopontin and osteocyte-derived factors in secondary hyperparathyroidism-induced myopathy

Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability

RORα overexpression reduced interleukin-33 expression and prevented mast cell degranulation and inflammation by inducing autophagy in allergic rhinitis

Activation of autophagy after blast-induced traumatic brain injury in mice

LncRNA THRIL, transcriptionally activated by AP-1 and stabilized by METTL14-mediated m6A modification, accelerates LPS-evoked acute injury in alveolar epithelial cells

Buyang Huanwu Decoction alleviates cerebral ischemic injury through modulating caveolin-1-mediated mitochondrial quality control

RNF113A targeted by miR-197 promotes proliferation and inhibits autophagy via CXCR4/CXCL12/AKT/ERK/Beclin1 axis in cervical cancer

Strontium Ranelate Inhibits Osteoclastogenesis through NF-κB-Pathway-Dependent Autophagy

ACKR4a induces autophagy to block NF-κB signaling and apoptosis to facilitate Vibrio harveyi infection

Sodium Butyrate Attenuates AGEs-Induced Oxidative Stress and Inflammation by Inhibiting Autophagy and Affecting Cellular Metabolism in THP-1 Cells

Regulation of the autophagy plays an important role in acute kidney injury induced acute lung injury

CAV1 alleviated CaOx stones formation via suppressing autophagy-dependent ferroptosis

eIF3k inhibits NF-κB signaling by targeting MyD88 for ATG5-mediated autophagic degradation in teleost fish

Endogenous dipeptide-carnosine supplementation ameliorates hypobaric hypoxia-induced skeletal muscle loss via attenuating endoplasmic reticulum stress response and maintaining proteostasis

The Protein-Independent Role of Phosphate in the Progression of Chronic Kidney Disease

Autophagic Degradation of Gasdermin D Protects against Nucleus Pulposus Cell Pyroptosis and Retards Intervertebral Disc Degeneration In Vivo

Hydrogen sulfide exacerbated periodontal inflammation and induced autophagy in experimental periodontitis

Exogenous Hydrogen Sulfide Ameliorates Diabetic Myocardial Fibrosis by Inhibiting Cell Aging Through SIRT6/AMPK Autophagy

MicroRNA-375 exacerbates knee osteoarthritis through repressing chondrocyte autophagy by targeting ATG2B

Galectin-3 mediates pulmonary vascular endothelial cell dynamics via TRPC1/4 under acute hypoxia

MicroRNA 30a Mediated Autophagy and Imatinib Response in Egyptian Chronic Myeloid Leukemia Patients

PTEN-Induced Putative Kinase 1 (PINK1)/Parkin-Mediated Mitophagy Protects PC12 Cells Against Cisplatin-Induced Neurotoxicity

Dopamine-melanin nanoparticles scavenge reactive oxygen and nitrogen species and activate autophagy for osteoarthritis therapy

Compressive force-induced autophagy in periodontal ligament cells downregulates osteoclastogenesis during tooth movement

miRNA-335-5p relieves chondrocyte inflammation by activating autophagy in osteoarthritis

Melatonin Enhances Proliferation and Modulates Differentiation of Neural Stem Cells Via Autophagy in Hyperglycemia

Association of astragaloside IV-inhibited autophagy and mineralization in vascular smooth muscle cells with lncRNA H19 and DUSP5-mediated ERK signaling

Recombinant Newcastle disease virus expressing human IFN-λ1 (rL-hIFN-λ1)-induced apoptosis of A549 cells is connected to endoplasmic reticulum stress pathways

Umbilical Cord Mesenchymal Stem Cells Conditioned Medium Promotes Aβ25-35 phagocytosis by Modulating Autophagy and Aβ-Degrading Enzymes in BV2 Cells

Expression of miR-551b and its effect on apoptosis in human gastric carcinoma

Lactone Component From Ligusticum chuanxiong Alleviates Myocardial Ischemia Injury Through Inhibiting Autophagy

Andrographolide Induces Autophagic Cell Death and Inhibits Invasion and Metastasis of Human Osteosarcoma Cells in An Autophagy-Dependent Manner

Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy resulting from enterovirus 71 and coxsackievirus A16 infections facilitates their replication

Tetrahydrocurcumin induces mesenchymal-epithelial transition and suppresses angiogenesis by targeting HIF-1α and autophagy in human osteosarcoma

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