Anti-Histidine decarboxylase/HDC Antibody
Rabbit IgG polyclonal antibody for Histidine decarboxylase(HDC) detection. Tested with WB, IHC-P in Mouse;Rat. Cited in 2 publication(s).
Anti-Histidine decarboxylase/HDC Antibody Info At A Glance
|Product Name||Anti-Histidine decarboxylase/HDC Antibody
See all Hdc primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for HDC detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. HDC information: Molecular Weight: 74045 MW; .|
|Cite This Product||Anti-Histidine decarboxylase/HDC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1703)|
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|Anti-Histidine decarboxylase/HDC Antibody may replace the following items: sc 34460|sc 34457|sc 34459|sc 34458|sc 68940|sc 34454|sc 34456|sc 34455.|
|Predicted Reactivity||Bovine, Canine, Chicken, Human, Xenopus
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse Histidine decarboxylase(620-639aa EQMMMMKKGAFKKLIKFYSV), different from the related rat sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
Protein Target Info (Source: Uniprot.org)
|Protein Name||Histidine decarboxylase(HDC)|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Catalyzes the biosynthesis of histamine from histidine. .|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Our Boster Quality Guarantee for Anti-Histidine decarboxylase/HDC Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
IHC(P): Rat Brain Tissue
HDC was detected in paraffin-embedded section of mouse brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1Î¼g/ml rabbit anti- HDC Antibody (PA1703) overnight at 4Â°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37Â°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Liu C, Chen J, Guo Y, Yang L, Zhao C, Bai L. Dig Dis Sci. 2011 Apr;56(4):993-8. Doi: 10.1007/S10620-010-1375-2. Epub 2010 Sep 16. The Expression Of Pthlh In Human Gastric Mucosa Enterochromaffin-Like Cells.||PubMed ID 20844958|
|The RNA-binding protein Celf6 is highly expressed in diencephalic nuclei and neuromodulatory cell populations of the mouse brain||PubMed ID 25682262|
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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