Click image to see more details
Product Info Summary
| SKU: | PB9376 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-HLA A/HLA-A Antibody Picoband®
SKU/Catalog Number
PB9376
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLA A/HLA-A Antibody Picoband® catalog # PB9376. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA A/HLA-A Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9376)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HLA A.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9376 is reactive to HLA-A in Human
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.8 kDa
Background of HLA-A
HLA-A, namely HLA A, is a group of human leukocyte antigens (HLA) that are coded for by the HLA-A locus, which is located at human chromosome 6p21.3. HLA is simply the major histocompatibility complex (MHC) specific to humans. HLA-A is one of three major types of human MHC class I cell surface receptors. The others are HLA-B and HLA-C. And it is critical to the cytotoxic t-cell controlled immune response to viruses and other intracellular pathogens. Because each HLA-A gene has a high affinity for slightly different peptides, certain HLA-As are associated with increased risk, more rapid progression, and/or increased severity of many diseases. For similar reasons, HLA-A matching is essential to successful tissue transplants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9376 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Positive Control
WB: human U87 whole cell, human HL-60 whole cell, human A431 whole cell, human HEL whole cell
IHC: Human Intestinal Cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HLA A using anti-HLA A antibody (PB9376).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U87 whole cell lysates,
Lane 2: human HL-60 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA A antigen affinity purified polyclonal antibody (Catalog # PB9376) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA A at approximately 41 kDa. The expected band size for HLA A is at 41 kDa.
Click image to see more details
IHC analysis of HLA A using anti-HLA A antibody (PB9376).
HLA A was detected in a paraffin-embedded section of Human Intestinal Cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-HLA A Antibody (PB9376) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Signaling pathways and genes altered in CRC progression. (A) Changes in signaling pathways as CRC developed in sample S115 by GSVA analysis. (B) Spatial distribution and expression trend of ribosome pathway in S115. (C) RPL5 (left) and IMP3 (right) expression gradually increased in the direction of the red arrow. (D) Spatial distribution and expression trend of antigen processing and presentation pathway in S115. (E) HLA-A (left) and HSPA2 (right) expression gradually increased in the direction of the blue arrow. (F, G) Expression direction of peroxisome pathway (F) and leucine and isoleucine degradation pathway (G) in S115. (H, I) Immunohistochemical staining showed expression of RPL5 (H) and HLA-A (I) in Mucosa and cancer(up) and Invasive margin(down) (n=45). The scale bars on the lower right in (H) , (I) are 100 µm.
Index in PubMed under a CC BY license. PMID: 36816947
Specific Publications For Anti-HLA A/HLA-A Antibody Picoband® (PB9376)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-HLA A/HLA-A Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-HLA A/HLA-A Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-HLA A/HLA-A Antibody Picoband®
Question
Is this PB9376 anti-HLA A/HLA-A antibody reactive to the isotypes of HLA-A?
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
The immunogen of PB9376 anti-HLA A/HLA-A antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human HLA A (82-106aa EYWDGETRKVKAHSQTHRVDLGTLR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-07
Question
Is there a BSA free version of anti-HLA A/HLA-A antibody PB9376 available?
Verified Customer
Verified customer
Asked: 2020-04-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-HLA A/HLA-A antibody PB9376 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-24
Question
Does PB9376 anti-HLA A/HLA-A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It shows on the product datasheet, PB9376 anti-HLA A/HLA-A antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
I see that the anti-HLA A/HLA-A antibody PB9376 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2020-02-20
Question
We have observed staining in mouse brain. What should we do? Is anti-HLA A/HLA-A antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-02-13
Answer
From literature brain does express HLA-A. From Uniprot.org, HLA-A is expressed in blood, brain, liver, cervix carcinoma, among other tissues. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2020-02-13
Question
I have attached the WB image, lot number and protocol we used for brain using anti-HLA A/HLA-A antibody PB9376. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-13
Question
Does anti-HLA A/HLA-A antibody PB9376 work for WB with brain?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
According to the expression profile of brain, HLA-A is highly expressed in brain. So, it is likely that anti-HLA A/HLA-A antibody PB9376 will work for WB with brain.
Boster Scientific Support
Answered: 2019-09-23
Question
We have tried in the past anti-HLA A/HLA-A antibody for WB on blood in the past. I am using rat, and We want to use the antibody for IHC next. We need examining blood as well as brain in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
I looked at the website and datasheets of our anti-HLA A/HLA-A antibody and it appears that PB9376 has been tested on rat in both WB and IHC. Thus PB9376 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-11
Question
We were happy with the WB result of your anti-HLA A/HLA-A antibody. However we have observed positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is HLA-A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-06-04
Answer
Based on literature, cervix carcinoma does express HLA-A. Generally HLA-A expresses in cell membrane. Regarding which tissues have HLA-A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 7836067, 8026990
Brain, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 23186163
Liver, Pubmed ID: 19159218, 24275569
Boster Scientific Support
Answered: 2019-06-04
Question
Is a blocking peptide available for product anti-HLA A/HLA-A antibody (PB9376)?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
We do provide the blocking peptide for product anti-HLA A/HLA-A antibody (PB9376). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-20
Question
I have a question about product PB9376, anti-HLA A/HLA-A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-06
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9376 anti-HLA A/HLA-A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-06
Question
We are currently using anti-HLA A/HLA-A antibody PB9376 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-02-08
Answer
The anti-HLA A/HLA-A antibody (PB9376) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-02-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-HLA A/HLA-A antibody PB9376. Let me know if you need anything else.
T. Krishna
Verified customer
Asked: 2017-09-22
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-22
Question
I was wanting to use your anti-HLA A/HLA-A antibody for WB for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
A. Moore
Verified customer
Asked: 2014-07-21
Answer
It shows on the product datasheet, PB9376 anti-HLA A/HLA-A antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-21
Question
We want to test anti-HLA A/HLA-A antibody PB9376 on rat brain for research purposes, then I may be interested in using anti-HLA A/HLA-A antibody PB9376 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Krishna
Verified customer
Asked: 2014-04-14
Answer
The products we sell, including anti-HLA A/HLA-A antibody PB9376, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-04-14
