Product Info Summary
| SKU: | PA2136-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CD134/OX40/TNFRSF4 Antibody Picoband®
SKU/Catalog Number
PA2136-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD134/OX40/TNFRSF4 Antibody catalog # PA2136-3. Tested in WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD134/OX40/TNFRSF4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2136-3)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of rat CD134.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2136-3 is reactive to Tnfrsf4 in Rat
Observed Molecular Weight
29 kDa
Calculated molecular weight
29.9 kDa
Background of Tnfrsf4
Tumor necrosis factor receptor superfamily, member4, also known as ACT35 or CD134 is a cell surface glycoprotein that was discovered through the production of a monoclonal antibody raised against the HUT-102 cell line. It belongs to the tumor necrosis factor receptor superfamily. CD134 was mapped to 1p36 by fluorescence in situ hybridization. CD134 is the primary receptor for feline immunodeficiency virus. CD134 expression can promote viral binding and renders cells permissive for viral entry, productive infection, and syncytium formation. Stimulating the receptor can improve the response to a powerful virus vector and may be useful in vaccine development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2136-3 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Rat
Positive Control
WB: Rat Brain Tissue, Rat Liver Tissue, Rat Kidney Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-CD134/OX40 antibody, PA2136-3, Western blotting
All lanes: Anti CD134/OX40 (PA2136-3) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 29KD
Observed bind size: 29KD
Specific Publications For Anti-CD134/OX40/TNFRSF4 Antibody Picoband® (PA2136-3)
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5 Customer Q&As for Anti-CD134/OX40/TNFRSF4 Antibody Picoband®
Question
I was wanting to use your anti-CD134/OX40/TNFRSF4 antibody for WB for rat brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat brain identification?
J. Thomas
Verified customer
Asked: 2020-01-14
Answer
As indicated on the product datasheet, PA2136-3 anti-CD134/OX40/TNFRSF4 antibody has been validated for WB on rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-14
Question
I have attached the WB image, lot number and protocol we used for brain using anti-CD134/OX40/TNFRSF4 antibody PA2136-3. Please let me know if you require anything else.
N. Evans
Verified customer
Asked: 2019-01-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-08
Question
Will PA2136-3 anti-CD134/OX40/TNFRSF4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Wu
Verified customer
Asked: 2017-11-23
Answer
It shows on the product datasheet, PA2136-3 anti-CD134/OX40/TNFRSF4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-23
Question
Is this PA2136-3 anti-CD134/OX40/TNFRSF4 antibody reactive to the isotypes of TNFRSF4?
O. Evans
Verified customer
Asked: 2017-01-24
Answer
The immunogen of PA2136-3 anti-CD134/OX40/TNFRSF4 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of rat CD134(23-42aa KLNCVKDTYPSGHKCCRECQ). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-01-24
Question
I am looking for to test anti-CD134/OX40/TNFRSF4 antibody PA2136-3 on rat brain for research purposes, then I may be interested in using anti-CD134/OX40/TNFRSF4 antibody PA2136-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
J. Evans
Verified customer
Asked: 2015-08-11
Answer
The products we sell, including anti-CD134/OX40/TNFRSF4 antibody PA2136-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-11


