Human TNFRSF4/OX40 ELISA Kit PicoKine™

Human TNFRSF4/OX40 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1106
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TNFRSF4/OX40 ELISA Kit PicoKine™

See all OX40/TNFRSF4 products

SKU/Catalog Number

EK1106

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TNFRSF4/OX40 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TNFRSF4/OX40 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1106)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: L29-A216

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1106 is reactive to TNFRSF4 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1106 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1106-CAP Anti-Human TNFRSF4 Pre-coated 96-well strip microplate 1
EK1106-ST Human TNFRSF4 Standard 2 vials, 10 ng/tube
EK1106-DA Human TNFRSF4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)02000200020002000200020002000
O.D.0.0030.0360.0810.1770.3450.7521.3212.314

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect OX40/TNFRSF4, Dilution ratio of 1:1, concentration in serum is NO-20pg/ml.

Some articles we found to cite concentrations of OX40/TNFRSF4 in samples: 18843780 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TNFRSF4/OX40 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7723075574223769
Standard deviation3.9213.5758.898.6%16.7266.13
CV (%)8.6%%7.8%%7.8%6.9%7.5%8.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of OX40/TNFRSF4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 177786778754.636.1%
Sample 223020220519920912.35.8%
Sample 375577179189180252.946.6%
*number of samples for each test n=16.

Gene/Protein Information For TNFRSF4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P43489

Gene ID

7293

Gene Name

TNFRSF4

Full Name

Tumor necrosis factor receptor superfamily member 4

Weight

29.341kDa

Alternative Names

ACT-135; ACT35 antigen; ACT35ATC35 antigen; CD134 antigen; CD134; Ly-70; OX40 cell surface antigen; OX40 homologue; OX40; OX40L receptor; OX40lymphoid activation antigene ACT35; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; TNFRSF4; tumor necrosis factor receptor superfamily member 4; tumor necrosis factor receptor superfamily, member 4; Txgp1; TXGP1L; TXGP1LOX40 antigen TNFRSF4 ACT35, CD134, IMD16, OX40, TXGP1L TNF receptor superfamily member 4 tumor necrosis factor receptor superfamily member 4|ACT35 antigen|ATC35 antigen|CD134 antigen|OX40 antigen|OX40 cell surface antigen|OX40 homologue|OX40L receptor|TAX transcriptionally-activated glycoprotein 1 receptor|lymphoid activation antigene ACT35|tax-transcriptionally activated glycoprotein 1 receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFRSF4, check out the TNFRSF4 Infographic

TNFRSF4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFRSF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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10 Customer Q&As for Human TNFRSF4/OX40 ELISA Kit PicoKine™

Question

Q: how to analyze ELISA data? I have obtained OX40 level in cell lysates.

Verified Customer

Verified customer

Asked: 2020-05-30

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-05-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Krishna

Verified customer

Asked: 2020-02-20

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-20

Question

Could you please give me more information on how EK1106 was tested on IHC-P and the specific antigen retrieval was used?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The EK1106 was tested in staining of paraffin embedded formalin fixed tissues using sodium citrate buffer (10 mM, pH6) for about 15 minutes for antigen retrieval. It also been successfully tested to stain dog lymph node tissue of formalin fixed paraffin embedded tissue sections again using citrate buffer as the antigen retrieval agent.

Boster Scientific Support

Answered: 2019-09-03

Question

Q: can you suggest the dilution ratio of serum samples for detection of OX40 in Human cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-07-07

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the OX40 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-07-07

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

T. Lopez

Verified customer

Asked: 2018-05-28

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-05-28

Question

Q: if the enzyme conjugated OX40 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the OX40 antigen?

E. Perez

Verified customer

Asked: 2018-04-22

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-22

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis OX40 using the EK1106 Human OX40 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-02-24

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-02-24

Question

Q: Can OX40 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

C. Turner

Verified customer

Asked: 2017-04-24

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-04-24

Question

Q: is it okay to use citrate plasma as samples in Human OX40 Picokine® ELISA Kit (Catalog # EK1106)?

B. Dhar

Verified customer

Asked: 2014-06-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of OX40 causing degradation of its protein structure. OX40 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the OX40 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2014-06-28

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

S. Kumar

Verified customer

Asked: 2014-02-19

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-02-19

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