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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-High mobility group protein B4 HMGB4 Antibody
Boster Bio Anti-High mobility group protein B4 HMGB4 Antibody catalog # PA1042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-High mobility group protein B4 HMGB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1042)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4 (24-41aa RNKFKEQQPNTYVGFKEF), different from the related mouse and rat sequences by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
No cross reactivity with other proteins
PA1042 is reactive to HMGB4 in Human, Mouse, Rat
PA1042 is guaranteed for WB Boster Guarantee
Background of HMGB4
HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Validation Images & Assay Conditions
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Anti-HMGB4 antibody, PA1042, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
Gene/Protein Information For HMGB4 (Source: Uniprot.Org, NCBI)
High mobility group protein B4
dJ1007G16.5; FLJ40388; high mobility group box 4; high mobility group protein B4; HMG2 like; MGC88128 HMGB4 dJ1007G16.5 high mobility group box 4 high mobility group protein B4|HMG2 like*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on HMGB4, check out the HMGB4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for HMGB4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
PA1042 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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