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Product Info Summary
| SKU: | PA1042 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-High mobility group protein B4 HMGB4 Antibody Picoband®
SKU/Catalog Number
PA1042
BA2191-2 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-High mobility group protein B4 HMGB4 Antibody catalog # PA1042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-High mobility group protein B4 HMGB4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1042)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4, different from the related mouse and rat sequences by one amino acid.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1042 is reactive to HMGB4 in Human, Mouse, Rat
Observed Molecular Weight
23 kDa
Calculated molecular weight
22.5 kDa
Background of HMGB4
HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1042 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Brain Tissue, Rat Testis Tissue, JURKAT Cell, HT1080 Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-HMGB4 antibody, PA1042, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
Lane 3: JURKAT Cell Lysate
Lane 4: HT1080 Cell Lysate
Specific Publications For Anti-High mobility group protein B4 HMGB4 Antibody Picoband® (PA1042)
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7 Customer Q&As for Anti-High mobility group protein B4 HMGB4 Antibody Picoband®
Question
We are currently using anti-HMGB4 antibody PA1042 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
The anti-HMGB4 antibody (PA1042) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-16
Question
I was wanting to use your anti-HMGB4 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
As indicated on the product datasheet, PA1042 anti-HMGB4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-04
Question
Does PA1042 anti-HMGB4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
It shows on the product datasheet, PA1042 anti-HMGB4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-28
Question
Is a blocking peptide available for product anti-HMGB4 antibody (PA1042)?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
We do provide the blocking peptide for product anti-HMGB4 antibody (PA1042). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-06-20
Question
I see that the anti-HMGB4 antibody PA1042 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-07-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-07-18
Question
Does anti-HMGB4 antibody PA1042 work for WB with testis?
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
According to the expression profile of testis, HMGB4 is highly expressed in testis. So, it is likely that anti-HMGB4 antibody PA1042 will work for WB with testis.
Boster Scientific Support
Answered: 2018-02-09
Question
See below the WB image, lot number and protocol we used for testis using anti-HMGB4 antibody PA1042. Please let me know if you require anything else.
A. Zhao
Verified customer
Asked: 2014-01-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-01-17
