Anti-High mobility group protein B4 HMGB4 Antibody

HMGB4 antibody

Boster Bio Anti-High mobility group protein B4 HMGB4 Antibody catalog # PA1042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1042
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-High mobility group protein B4 HMGB4 Antibody

View all HMGB4 Antibodies

SKU/Catalog Number



100 μg/vial




Boster Bio Anti-High mobility group protein B4 HMGB4 Antibody catalog # PA1042. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-High mobility group protein B4 HMGB4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1042)




Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.




Rabbit IgG


A synthetic peptide corresponding to a sequence at the N-terminus of human HMGB4 (24-41aa RNKFKEQQPNTYVGFKEF), different from the related mouse and rat sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.


No cross-reactivity with other proteins

Reactive Species

PA1042 is reactive to HMGB4 in Human, Mouse, Rat


PA1042 is guaranteed for WB Boster Guarantee

Background of HMGB4

HMGB4 is HMG2 like, isoform 2, high-mobility group box 4. This gene can be found on Chromosome 1. HMGB4 contains two HMG-box regions, which is found in a variety of eukaryotic chromosomal proteins and transcription. HMGB proteins are phosphorylated to various extents reveals that the existence of differentially modified forms increases the number of distinct HMGB protein variants in plant chromatin that may be adapted to certain functions.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Validation Images & Assay Conditions

Gene/Protein Information For HMGB4 (Source:, NCBI)

Uniprot ID


Gene ID


Gene Name


Full Name

High mobility group protein B4




HMGB family

Alternative Names

dJ1007G16.5; FLJ40388; high mobility group box 4; high mobility group protein B4; HMG2 like; MGC88128 HMGB4 dJ1007G16.5 high mobility group box 4 high mobility group protein B4|HMG2 like

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HMGB4, check out the HMGB4 Infographic

HMGB4 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HMGB4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]

PA1042 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

HMGB1, TGF-? and NF-?B are associated with chronic allograft nephropathy

HMGB4 is expressed by neuronal cells and affects the expression of genes involved in neural differentiation

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7 Customer Q&As for Anti-High mobility group protein B4 HMGB4 Antibody


We are currently using anti-HMGB4 antibody PA1042 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-16


The anti-HMGB4 antibody (PA1042) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-16


I was wanting to use your anti-HMGB4 antibody for WB for mouse testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse testis identification?

Verified Customer

Verified customer

Asked: 2019-11-04


As indicated on the product datasheet, PA1042 anti-HMGB4 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse testis in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-11-04


Does PA1042 anti-HMGB4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-06-28


It shows on the product datasheet, PA1042 anti-HMGB4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-06-28


Is a blocking peptide available for product anti-HMGB4 antibody (PA1042)?

Verified Customer

Verified customer

Asked: 2019-06-20


We do provide the blocking peptide for product anti-HMGB4 antibody (PA1042). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-06-20


I see that the anti-HMGB4 antibody PA1042 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-07-18


You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-07-18


Does anti-HMGB4 antibody PA1042 work for WB with testis?

Verified Customer

Verified customer

Asked: 2018-02-09


According to the expression profile of testis, HMGB4 is highly expressed in testis. So, it is likely that anti-HMGB4 antibody PA1042 will work for WB with testis.

Boster Scientific Support

Answered: 2018-02-09


See below the WB image, lot number and protocol we used for testis using anti-HMGB4 antibody PA1042. Please let me know if you require anything else.

A. Zhao

Verified customer

Asked: 2014-01-17


Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-01-17



Total: $315


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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)

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