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Product Info Summary
| SKU: | PA1505 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bone Sialoprotein/IBSP Antibody Picoband®
SKU/Catalog Number
PA1505
BA2329 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bone Sialoprotein/IBSP Antibody catalog # PA1505. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bone Sialoprotein/IBSP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1505)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein, different from the related rat and mouse sequences by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1505 is reactive to IBSP in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Calculated molecular weight
35.1 kDa
Background of IBSP
BSP is also known as IBSP (integrin-binding sialoprotein). The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1505 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
IHC: Human Osteosarcoma Tissue
Validation Images & Assay Conditions
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Anti-Bone Sialoprotein antibody, PA1505, IHC(P)
IHC(P): Human Osteosarcoma Tissue
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Analysis of the mRNA and protein expression of osteogenic differentiation markers during the osteogenic differentiation of DFCs. a Histograms showing the relative mRNA levels of ALP, BSP, OCN , and RUNX2 during the induction of differentiation. The mRNA levels were determined using RT-PCR with GAPDH as a reference for normalization. b Representative western blots showing the protein levels of ALP, BSP, OCN, and RUNX2. c Histograms showing the relative protein levels of ALP, BSP, OCN, and RUNX2 during the induction of differentiation. Values are means ± SD of three independent experiments (* P < 0.05 and ** P < 0.01)
Index in PubMed under a CC BY license. PMID: 32737282
Specific Publications For Anti-Bone Sialoprotein/IBSP Antibody Picoband® (PA1505)
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