Anti-Granzyme A/GZMA Antibody
Rabbit IgG polyclonal antibody for Granzyme A(GZMA) detection. Tested with WB, IHC-P in Human.
Anti-Granzyme A/GZMA Antibody Info At A Glance
CBX5 belongs to the heterochromatin protein family. CBX5 is enriched in the heterochromatin and linked with centromeres. CBX5 takes part in the creation of functional kinetochore by interaction with important kinetochore proteins. CBX5 has a pseudogene situated on chromosome 3. Additionaly, The CBX5 proteins reconnect with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.
|Product Name||Anti-Granzyme A/GZMA Antibody
See all Granzyme A primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Granzyme A/GZMA detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Granzyme A/GZMA information: Molecular Weight: 28999 MW; Subcellular Localization: Isoform alpha: Secreted. Cytoplasmic granule.|
|Cite This Product||Anti-Granzyme A/GZMA Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1588)|
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|Anti-Granzyme A/GZMA Antibody may replace the following items: sc 11434|sc 5510|sc 5511|sc 56115|sc 56116.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Granzyme A(175-190aa DRKVCNDRNHYNFNPV).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For GZMA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||3001|
|Species Of This Entry||Human|
|Protein Name||Granzyme A|
|Superfamily||peptidase S1 family|
|Alternative Names||Granzyme A|CTL Tryptase; CTLA3; CTLA3Granzyme A (Cytotoxic T-lymphocyte-associated serine esterase-3; Hanukah factorserine protease); Cytotoxic T-lymphocyte proteinase 1; EC 3.4.21; Fragmentin-1; granzyme A (granzyme 1, cytotoxic T-lymphocyte-associated serine esterase 3); Granzyme A; Granzyme-1; GZMA; H factor; Hanukkah factor; HF; HFSP; HFSPEC 22.214.171.124|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Myristoylation; Sulphation; Reduction; Glycosylation; Oxidation; Methylation; Prenylation; Ribosylation|
|Subcellular Localization||[Isoform alpha]: Secreted. Cytoplasmic granule.|
|Gene Location||5q11.2, on Chromasome 5, gene sequence: NC_000005.10 (55102646..55110252)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For GZMA (Source: Uniprot.org, NCBI)
|Protein Function||Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent cell death with morphological features of apoptosis when delivered into the target cell through the immunological synapse. It cleaves after Lys or Arg. Cleaves APEX1 after 'Lys-31' and destroys its oxidative repair activity. Cleaves the nucleosome assembly protein SET after 'Lys-189', which disrupts its nucleosome assembly activity and allows the SET complex to translocate into the nucleus to nick and degrade the DNA.|
|Related Diseases||Neoplasms; Infective Disorder; Virus Diseases; Leukemia; Rheumatoid Arthritis; Hiv Infections; Malignant Neoplasms; Arthritis; Immunologic Deficiency Syndromes; Inflammation; Tissue Adhesions; Autoimmune Reaction; Lymphoma; Cytomegalovirus Infections; Degenerative Polyarthritis; Melanoma; Decreased Immunologic Activity [pe]; Neoplasm Metastasis; Sjogren's Syndrome; Graft-vs-host Disease|
|Related Pathways||Cell Death; Exocytosis; Secretion; Cytolysis; Pathogenesis; Immune Response; Proteolysis; Cell Proliferation; Cell Killing; Programmed Cell Death; Induction Of Apoptosis; Localization; Nucleosome Assembly; Viral Replication; Cell Activation; Cell Differentiation; Cell Recognition; Reverse Transcription; Angiogenesis; Killer Activity|
|Background||Granzyme A is a protein that in humans is encoded by the GZMA gene. Cytolytic T lymphocytes(CTL) and natural killer(NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are though to protect their host by lysing cells bearing on their surface "nonself" antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here is a T cell- and natural killer cell-specific serine protease that may function as a common component necessary for lysis of target cells by cytotoxic T lymphocytes and natural killer cells. GZMA induces caspase-independent apoptosis in a characteristic manner, except it causes a distinctive form of DNA damage: single-stranded DNA nicking. A target of GZMA is the SET complex, including HMGB2 and ANP32A .|
|Scientific References||PMID: 3257574 by Gershenfeld H.K., et al. Cloning and chromosomal assignment of a human cDNA encoding a T cell- and natural killer cell-specific trypsin-like serine protease.|
PMID: 17180578 by Ruike Y., et al. Glucocorticoid-induced alternative promoter usage for a novel 5' variant of granzyme A.
Our Boster Quality Guarantee for Anti-Granzyme A/GZMA Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
IHC(P): Human Tonsil Tissue
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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